In a recent publication, researchers from the University of Miami Miller School of Medicine (USA) describe that Serotonin released by human beta cell inhibits glucagon secretion by alpha cells. They demonstrated that this paracrine loop was mediated via the cAMP pathway. To do so, they captured in live human pancreatic islet cells cAMP signals using a specific fluorescent biosensor.
In drug discovery screening campaigns as well as in fundamental research activities, cellular parameters have to be measured and monitored in living cells regularly. Often fluorescent dyes are used to detect and follow some of the parameters. These methods are powerful, especially in screening large numbers of compounds, but have their limitations e.g. when it comes to measuring more than one parameter in the same living cell, or when specific locations in the cell shall be targeted.
To overcome these limitations and to provide tools to especially look into GPCR related signalling in the most comprehensive and detailed way currently possible, Montana Molecular have developed genetically encoded fluorescent biosensors to measure parameters such as cAMP, DAG, PIP2, Ca2+and voltage changes in living cells. [Read more…]