High quality isolation of lymphocytes is a key step in many in vitro research programs covering immunology, infectious diseases and oncology. To simplify this step, CEDARLANE® have developed an efficient and easy-to -use cell separation media: Lympholyte®
The complement system helps the ability of antibodies and phagocytic cells to clear pathogens from an organism. It is part of the innate immune system that is not adaptable and does not change over the course of an individual’s lifetime. However, it can be recruited and brought into action by the adaptive immune system. The complement system is also used in research laboratories for in vitro assays during vaccine development, cytotoxicity testings… But how to select to most appropriate Rabbit or Guinea pig complement according to research applications and sample types?
Analysis of Autofluorescence in Polymorphonuclear Neutrophils: A New Tool for Early Infection Diagnosis by Monsel A et al.
In this study, the authors demonstrates that the average autofluorescence changes of the PolyMorphonuclear Neutrophils (PMNs) during bacterial infections, can serve as a surrogate marker of infection. The autofluorescence is related to NAD(P)H pathway in PMNs.
Source: Monsel A et al. (2014) Analysis of Autofluorescence in Polymorphonuclear Neutrophils: A New Tool for Early Infection Diagnosis. PLoS ONE 9(3): e92564. doi:10.1371/journal.pone.0092564
Congratulations to the authors who have relied on tebu-bio when selecting their discovery tools.
Want to know more about Lympholyte to isolate human Polymorphonuclear Neutrophils?
Lympholyte-poly is a ready-made sterile and endotoxin tested solution which is a mixture of sodium metrizoate and Dextran 500 that allows for the isolation of human polymorphonuclear granulocytes from whole blood.
• Protein production • Plasmid Preps • 3D Cell Culture