Visualizing fixed cells and tissues only gives snapshots of cellular processes. To have a better insight into dynamic events ocurring in the cells or to vizualize interactions between various cellular components in real time (e.g., proteins, organelles, second messengers…), powerful microscopic approaches have been developed over the past decade. This post will review the recent live cell imaging probes developed by Goryo Chemicalsand available in Europe through tebu-bio.
In the autumn of 2014, we presented new stains to conduct live cell imaging of Actin and Tubulin, which I covered in my post 2 new Actin and Tubulin live-cell imaging stains – without transfection!
- No transfection
- No washing steps
- No toxic effects if used in the concentration range recommended
- Excellent brightness
- Far-red excitation & emission
- Deep tissue penetration and minimal background
- Multiple fluorescent stainings with other dyes possible
- Compatible with Superresolution microscopic techniques
Since then, Spirochrome has added another stain with the same benefits: SiR-DNA, a far-red, fluorogenic, cell permeable and highly specific probe for DNA (see Fig 1).
SLAS – High Content Screening Conference
From June 27th to June 29th, the SLAS – High Content Screening Conference took place in Dresden/Germany. The conference focussed on new essays and novel microscopy techniques.
Spirochrome and tebu-bio were selected to present a poster about the features of SiR-stains, which are excellent tools for phenotypic screening and high content screening (HCS) approaches.
If you are interested in learning more about these exciting live cell imaging tools, don’t hesitate to download our joint poster:
Any questions or comments? Please feel free to contact me with the form below!
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In a lot of experimental setups it would be eligible to be able to differentiate human ES and iPS cells from differentiated cells.
tebu-bio is pleased to announce their cooperation with Goryo Chemical, a company specialized in probes for Live Cell Imaging. Goryo Chemical recently launched the Kyoto probe 1 (KP-1) which exactly fulfills this need. [Read more…]
In drug discovery screening campaigns as well as in fundamental research activities, cellular parameters have to be measured and monitored in living cells regularly. Often fluorescent dyes are used to detect and follow some of the parameters. These methods are powerful, especially in screening large numbers of compounds, but have their limitations e.g. when it comes to measuring more than one parameter in the same living cell, or when specific locations in the cell shall be targeted.
To overcome these limitations and to provide tools to especially look into GPCR related signalling in the most comprehensive and detailed way currently possible, Montana Molecular have developed genetically encoded fluorescent biosensors to measure parameters such as cAMP, DAG, PIP2, Ca2+and voltage changes in living cells. [Read more…]
Launched just over a year ago, SiR-Actin (Fig 1) and SiR-Tubulin (Fig 2) have been available on the market providing the most convenient tools to stain F-actin and Microtubules in living cells. In the meantime, Spirochrome have launched a third stain based on the SiR-technology – SiR-DNA (Fig 3) to stain DNA in living cells.
These stains meet the central requirements for live cell imaging tools:
- high selectivity
- minimal toxicity
- fluorogenic for wash-free imaging
- applicable in different cell types and tissues
- excited by far-red light
- suitable for super-resolution microscopy
Visualizing fixed cells and tissues only gives snap shots of cellular processes. Over the past years, more and more cellular parameters have become measurable in living cells. For this purpose, there is a growing number of specific stains capable of entering the cell without toxic effects (which could also negatively impact the parameters to be measured). [Read more…]
Thousands of researchers spend time pampering their primary cells and cell lines every day, with the aim of defining their optimal cell culture conditions. Researchers are in effect trying to control numerous cellular parameters (oxygene levels, fresh media supply, incubation temperature…) and monitor their “viability” on a regular basis. But what about the internal cellular temperature? Have you ever considered this biological parameter to monitor the “wellness” of your cultivated cells? In this post, let’s take a look at the characteristics of a new, cell permeable, fluorescent thermometer for living cells.
In their most recent publication “SiR-Hoechst, a far-red DNA stain for live-cell nanoscopy” in Nature Communications, Lukinavičius et al. (1) define ideal characteristics of a nuclear stain for live cell imaging approaches. The stain should…
- show high selectivity
- minimal toxicity
- be fluorogenic for wash-free imaging
- be applicable in different cell types and tissues
- be excited by far-red light
- be suitable for super-resolution microscopy
None of the nuclear stains on the market meets all these crucial requirements leading thus to the need for a new tool for live cell staining experiments of the nucleus. [Read more…]
Recently, a we published a series of posts focusing on research tools aiming at studying actin through biochemical assays and in fixed and living cells (e.g. Actin visualization, Actin binding, Actin polymerization, and measurement of the G:F actin ratio in cells).
Today, this is the first post on how to vizualize tubulin in cells. This is the first publication of a series, that will be dedicated to tubulin visualization, binding and polymerization as well as measurement of the tubulin vs. microtubule ratio.