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How to easily visualize and quantify cellular senescence

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Due to its involvement in many cellular processes such as normal ageing, tumor suppression, chronic diseases and many more, cellular senescence has gained increasing attention since its first description over 50 years ago. In addition, understanding of cellular senescence is invaluable in the development of therapeutic targets in the fight against age-related co-morbidities, cancer and chronic disease progression.

Over-expressed in senescent cells, Senescence-associated β-galactosidase (SA-β-gal), has been widely used as a marker of cellular senescence. In order to specifically detect this biomarker directly in living cells or tissues, let’s take a look at an easy and ready-to-use assay recently developed: the SPiDER-βGal- Cellular Senescence Detection kit. [Read more…]

Live Cell Imaging – Monitor your cellular events by Fluorescent Microscopy

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Live Cell Imaging has transformed the way biologists study cellular functions and processes. The observation of dynamic changes provides more insight into the processes happening in a cell, as compared to imaging studies of fixed cells. Goryo Chemical have developed a broad range of innovative Live Cell Imaging fluorescent probes for various applications. Let’s take a closer look… [Read more…]

cAMP signals visualized in live human pancreatic primary cells

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In a recent publication, researchers from the University of Miami Miller School of Medicine (USA) describe that Serotonin released by human beta cell inhibits glucagon secretion by alpha cells. They demonstrated that this paracrine loop was mediated via the cAMP pathway. To do so, they captured in live human pancreatic islet cells cAMP signals using a specific fluorescent biosensor.

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Microcell arrays – obtain more accurate data from single living cells

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Understanding how mammalian cells function requires a dynamic perspective. Recent improvements in our abilities to perform fluorescence microscopy on primary cells, coupled with advances in pipelines for quantifying and extracting data, have made possible a better understanding of the temporal complexity of cell signalling pathway. Due to the heterogeneity seen in both eukaryotic and prokaryotic cell populations, study at the single cell resolution with living cells is currently the best solution to understand the dynamics between environmental conditions and cellular behaviour.

However as living cells don’t stay still, classical imaging and studying methods present some drawbacks for single cell analysis and tracking such as: [Read more…]

New HYDROP fluorescent dye for intracellular ROS measurement

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Reactive oxygen species (ROS) play key roles in various intracellular processes and have been shown to be involved in many diseases (eg. carcinogenesis, inflammation…). Each of the ROS species is likely to have a specific role in living cells. Therefore, there is an emerging need for selectively detecting each species of ROS through conventional biochemical assays, but also in live cell imaging (see a previous post “Reactive Oxygen Species (ROS) and related assay kits“).

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KP-1 dye distinguishes stem cells from differentiated cells by LCI and FCM

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Live cell imaging and cell sorting by flow cytometry are possible experimental approaches for researchers working in stem cell research and regenerative medicine. In this post, we take a look at the Kyoto Probe 1 (KP-1)  from (GORYO Chemicals).

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SiR fluorogenic probes: multicolour live-cell Imaging of Actin, Tubulin, DNA, and Lysosomes

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The Silicon Rhodamine-like (SiR) technology has significantly contributed to the recent development of DNA and cytoskeletal analysis by live cell imaging.

Spectrum SiR 655. Spirochrome - tebu-bio.In 2014, two new Silicon Rhodamine-like (SiR) fluorescent probes were released for studying actin & tubulin by live cell imaging. SiR-Actin and SiR-Tubulin are fluorescent probes compatible with most microscopes (including super-resolution settings) that directly stain actin & tubulin without the need to transfect cells with vectors expressing fluorescently labeled Actin or Tubulin. The two original dyes were successfully followed by a new SiR-DNA probe in order to visualize DNA in living cells.

The existing SiR stains have a λabs of 652 nm and a λem of 674 nm to be used with the Cy5 filter (Fig 1).

However, the continuously growing number of researchers using these stains asked us whether stains with different biophysical properties would be made available. In other words, they were asking “is there another colour to allow for double staining e.g. of Actin and Tubulin in living cells?”

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