With their polycationic properties, polyamine organic compounds are involved in various biological functions (DNA-RNA interaction, gene expression…), and can also be considered as cancer marker.

In this post, I’m pleased to introduce the first intra-cellular polyamine imaging reagent which works without any pre-treatment or cell lysis. This innovative reagent can be used for live cells, thus making it suitable for detection or semi-quantitative analysis of intra-cellular polyamines.

Glutathione (GSH) is a tri-peptide (Glutamic acid, Cystein and Glycine) present in cells, showing an antioxidant effect allowing the reduction and the removal of Reactive Oxygen Species (ROS) and Peroxide from the cell. The new QuicGSHS3.0 probe from Goryo Chemicals will allow you to easily quantify intracellular concentration of GSH by Fluorescent Live cell Imaging.

Due to its involvement in many cellular processes such as normal ageing, tumor suppression, chronic diseases and many more, cellular senescence has gained increasing attention since its first description over 50 years ago. In addition, understanding of cellular senescence is invaluable in the development of therapeutic targets in the fight against age-related co-morbidities, cancer and chronic disease progression.

Over-expressed in senescent cells, Senescence-associated β-galactosidase (SA-β-gal), has been widely used as a marker of cellular senescence. In order to specifically detect this biomarker directly in living cells or tissues, let’s take a look at an easy and ready-to-use assay recently developed: the SPiDER-βGal- Cellular Senescence Detection kit.

Live Cell Imaging has transformed the way biologists study cellular functions and processes. The observation of dynamic changes provides more insight into the processes happening in a cell, as compared to imaging studies of fixed cells. Goryo Chemical have developed a broad range of innovative Live Cell Imaging fluorescent probes for various applications. Let’s take a closer look…