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You are here: Home / Archives for Lipidomics

LipiDye fluorescent probe for lipid droplet monitoring in live cells

10/11/2016 By Philippe Fixe, PhD Leave a Comment

LipiDye lipid droplet Green* is a new highly sensitive fluorescent probe for detecting lipid droplets in live or fixed cells. In this, let’s review the characteristics of this dye which is compatible with live cell imaging. [Read more…]

Filed Under: Cell Biology and Signalling Tagged With: Lipidomics, Lipids, Live cell imaging

Lipid-Ligand interaction tools: lipids are not scary intermediates anymore

24/06/2014 By Paola Vecino, PhD 6 Comments

The first time a cell biologist discovers that there might be a lipid involved in their favourite pathway, it’s a bit like finding a wall across the road (just the same situation as when a lipidomist finds a protein in their way…).

Due to the different nature of lipids, most cell biologists fear having to face this situation along their way. The good news is that there are some simple methods to help overcome this obstacle, and make the studyof lipids in a given pathway easier. Here, I’d like to go over some analytical and preparative tools for lipid-ligand interaction studies.

[Read more…]

Filed Under: Cell Biology and Signalling Tagged With: Floatation assay, Lipid assays, Lipid interaction, Lipidomics, Liposomes, Overlay assay, Phosphoinositides, Pull-down assays

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