Lentiviruses are robust tools for cell line engineering and development. As opposed to models that only infect dividing cells, lentiviral vectors are ideal for long term gene delivery into dividing and quiescent cells. This post is aimed at introducing the newest evolutions of lentiviral experimental approaches: the 3rd generation lentiviral plasmids.
Over the last decade, Lentivirus progressively became a research tool for stable cell line engineering and production. What makes them so attractive?