Cardiovascular disease (CVD) comprehensively describes a range of pathologies of the heart and/or blood vessels. These disorders differ markedly in time-scale of development, and relative contributory effects of genes and environment. Largely due to this breadth, and their causes and effects, the range of investigation, assessment, and treatment strategies available is large. The diversity of the diseases, disease processes, and genetic differences between individuals could obscure disease associations in terms of the proteomic signatures of each disorder. Thus, a panel of independent disease-related proteins considered together should be less prone to this noise and help provide much more robust analysis and indication of disease. The publication featured below and the reagents (multi-plex immunoassays or single target ELISA and antibodies) mentioned in this post may contribute in helping you to identify such diseases signature.

With more than 170,000 publications in 2016, inflammation remains a “hot” topic in today’s Life Science research (source: Google Scholar). Interestingly, the ELISA test is still one of the most popular tools for the accurate quantification of human cytokines involved in this biological process. Here, we review the 5 protein targets most studied by our clients, together with their favourite ELISA kits by RayBiotech in the field of inflammation.

Typical Standard Curves of Our New PGE2 EIA Kit (Cat. number 183K051-H1).

PGE₂ is a potent lipid mediator involved in maintaining homeostasis but also in the promotion of acute inflammation or immune suppression in chronic inflammation and cancer. Recently, researchers have shown that the activation of the NLRP3 inflammasome in human primary monocyte-derived macrophages is inhibited by PGE₂ (1) confirming the role of this lipid is an effective autocrine and paracrine inflammatory modulator maintaining homeostasis during the resolution phase of inflammation.

This paper also proove the importance of having access to simple but robust PGE2 quatitative assays.

Which Prostaglandin E2 (PGE2) Immunoassay kit to choose?

The Prostaglandin E2 (PGE2) Immunoassay kit is designed to quantitatively measure PGE2 present in serum, plasma, urine, saliva, cells, tissue, and tissue culture media samples. This
EIA kit allows for the widest variations in sample size, sensitivity and assay timing of any PGE2 kit:

• Super Fast Kinetics – Same Assay Range at 2 or 16 hours
• Incredible Sensitivity – < 2 pg/mL
• Superior Cross Reactivity to Other Eicosanoids
• Super Low Sample Volume Requirements
• Choice of Incubation Time, Sensitivity, and Sample Volume in a Single Kit

Want to test this kit allowing you to accomplish any PGE₂ measurement you may have, do not hesitate to contact us!

References

1.- Sokolowska, M. et al. (2015). The Journal of Immunology, vol. 194 no. 11 5472-5487. doi: 10.4049/​jimmunol.1401343.

Chronic Mucosal Inflammation is the hallmark of common airway diseases (ex.  Allergic Rhinitis and asthma). Lipoxin B₄(LXB₄) is an endogenous mucosal protective mediator decreasing such diseases. LXB4 mechanisms of action remain poorly understood.

Cytokine Quantibody Arrays from Raybiotech and tebu-bio laboratories.

In a recent paper in Mucosal Immunology (Karra, L. et al. (2015) 8; DOI:10.1038/mi.2014.116), Allergic Rhinitis  and asthma murine models have been described to better investigate the role of LXB4 in Mucosal Inflammation. The authors conclude that, in the upper airway, LXB₄ significantly decreases nasal mucosal leukocytes and degranulation of Mast Cells (MCs) and Eosinophils. They also show that, in the lower airway, LXB₄ significantly decreased airway inflammation, mucus metaplasia, and hyper-responsiveness.