During the past decade, mass spectrometry, notably LC/MS, has become a major approach to identify and quantify proteins in patient samples. It includes analysis before treatments to find the proper biomarkers for cancers diagnostics, and monitoring of the arising of the anti-drug antibodies in human plasma after therapies. Despite the fact that MS has pretty high sensitivity and specificity, research in biotherapeutics requires even more. Immunocapture, and so specific protein enrichment, offer an interesting immunoassay solution. Here, we present simple to use and efficient reagents to improve the immunocapture and thus the output of the MS technology. [Read more…]
Protein multiplex profiling as simple as western blot
In one of my previous posts, I wrote about high density glass slide antibody arrays services.
Here, I’d like to focus on another antibody microarray product line, the C-series from Raybiotech, based on a membrane antibody array technology. Similar to western blotting – therefore very easy to process – they are suitable to most research laboratories for targeted protein profiling.
RayBiotech award tebu-bio as Best in Europe
RayBiotech 2017 Outstanding Distributor Award – tebu-bio Best in Europe.
At the recent meeting of all RayBiotech distributors, Isabelle Nobiron PhD (Product Specialist, tebu-bio), was proud to receive the 2017 Outstanding Distributor Award – Best in Europe.
During this meeting, which took place in Guangzhou (China), RayBiotech (Norcross, USA) also appointed tebu-bio as one of the 3 centers in the world to perform contract research for their high density arrays. Up to 1000 human proteins, 200 mouse and 67 rat biomarkers can be tested on semi-quantitative or quantitative proteomics microarray platforms.
tebu-bio has been a longstanding, recognised RayBiotech Certified Services provider, performing outsourced immunoassays at their European Headquarters situated near Paris (full range of services tebu-bio services here).
RayBiotech, Inc. develop cutting edge proteomics tools for the advancement of basic and clinical research, and the facilitation of drug and biomarker discovery. Their product-line covers 2,000+ rigorously tested ELISA tests (covered by a 90-day 100% satisfaction guarantee), Multiplex ELISA Arrays (Quantibody®), Membrane- or glass-slide Antibody Arrays (C-Series, G-Series, L-Series, E-Series), Functional assays, Research antibodies and Recombinant proteins.
tebu-bio provides their broad range of products to researchers across Europe. For more details, contact your local tebu-bio office.
6 ELISA test formats for protein target and biomarker discoveries
Having access to highly reproducible and fully validated ELISA tests is crucial in today’s Life Science research. Although the Sandwich ELISA format is the most commonly used, other immunoassay formats are also available to allow for a complete tool box, from phosphorylation studies to transcription factor activity assays.
In this post, I’ll be taking a look at the 6 formats available. [Read more…]
Analysis tool for direct and sandwich ELISA – convenient and reliable
Many ELISA kits – one analysis tool
Analyte quantification (in pg/ml or ng/ml) such as IL-6, TNFalpha and numerous other proteins is conveniently performed with immunoassays called ELISA kits. At tebu-bio, we not only provide more than 10 000 ELISA kits but we also support researchers through our techserv team who are on-hand to suggest the best kits and to answer any questions.
Being part of that team, I have noticed a number of often asked questions that can be resumed in one : What is the best method for data analysis? [Read more…]