By mimicking in-vivo environments, 3D cell culture models appear nowadays as the best in-vitro study model to work in an in-vivo like study model and to obtain more physiologically relevant data and proof of concept as close as possible to “a clinical context” (aka the “near-human” approach).
To accompany researchers along this way, there are several innovative cell culture devices (available in Europe through tebu-bio), and more specifically a modular microfluidic platform for 3D cell culture with the capability to monitor complex biological systems dynamics in response to tuned microenvironment: the 3D Cell culture chip (Aim Biotech)
For more than 2 years now, the Silicon Rhodamine-like (SiR) technology has allowed the live cell imaging field with fluorescence microscopy to evolve significantly.
Fluorescent SiR-probes have appeared as the best alternative tools for studying Actin (SiR-actin), Microtubules (SiR-Tubulin), DNA (SiR-DNA) and now lysosome (SiR-Lysosome) for live cell imaging. Who better to show this? Well, here’s how other researchers have been using them to get optimal results.