In a recent publication, researchers from the University of Miami Miller School of Medicine (USA) describe that Serotonin released by human beta cell inhibits glucagon secretion by alpha cells. They demonstrated that this paracrine loop was mediated via the cAMP pathway. To do so, they captured in live human pancreatic islet cells cAMP signals using a specific fluorescent biosensor.
Reactive oxygen species (ROS) play key roles in various intracellular processes and have been shown to be involved in many diseases (eg. carcinogenesis, inflammation…). Each of the ROS species is likely to have a specific role in living cells. Therefore, there is an emerging need for selectively detecting each species of ROS through conventional biochemical assays, but also in live cell imaging (see a previous post “Reactive Oxygen Species (ROS) and related assay kits“).
The detection of molecular events in living cells is booming. In this post, we look at 3 fluorescent probes that will undoubtedly count in the live-cell imaging landscape in 2017.