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Research areas
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tebu-bio's blog - Acting and reacting in life sciences and biotechnologies
  • Home
  • Research areas
    • ADME-Tox
    • Biomarkers
    • Cell Biology and Signalling
    • Cell Sourcing – Cell Culture Technologies
    • Drug Discovery
    • Gene Expression – Molecular Biology
    • Stem Cells
    • Supplying Discovery Tools
  • Contact us
  • Meet the authors
Cell Biology and Signalling

3 fluorescent probes that will count in 2017

02/12/2016 by Philippe Fixe, PhD No Comments
acidifluor-orange-multicolor-fluorescence-in-hela-cells

The detection of molecular events in living cells is booming. In this post, we look at 3 fluorescent probes that will undoubtedly count in the live-cell imaging landscape in 2017.

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Cell Biology and Signalling

KP-1 dye distinguishes stem cells from differentiated cells by LCI and FCM

28/11/2016 by Philippe Fixe, PhD 2 Comments
ips-colony-bright-field | tebu-bio

Live cell imaging and cell sorting by flow cytometry are possible experimental approaches for researchers working in stem cell research and regenerative medicine. In this post, we take a look at the Kyoto Probe 1 (KP-1)  from (GORYO Chemicals).

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Drug Discovery

What will the Next-Gen Protease activity detection be?

25/06/2015 by Philippe Fixe, PhD No Comments

Fluorescent technologies enable the measurement of protease activities and the screening of compounds influencing protease activities. Fluorescence Resonance Energy Transfer (FRET) but also TR-FRET, Q-FRET together with Time Resolved Fluorescence (TRF) and Fluorescence Lifetime (FLT) are popular assays, in which protease substrates occupy a central place. These assays are based on the specific recognition and cleavage of a peptide sequence (substrate) by the protease of interest. If the substrate is not optimally designed (or too short), experimental output can be unrelevant: low selectivity and specificity, high background, false negatives or positives…). In addition, the data can also be affected by the reaction buffer (pH, compound solvent…).This lack of relevant biological information is at the origin the emergence of “Next-Gen” fluorescent protease substrates.

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Supplying Discovery Tools

Flow cytometry intracellular staining

02/06/2015 by Elodie Monin No Comments

Intracellular Flow Cytometry enables the identification and analysis of signaling and functional markers within cells from samples containing heterogeneous cell populations. Many cell types can be thus identified through their intracellular Flow Cytometry patterns and key intracellular signaling pathways and their respective post-translational modifications can be conveniently analysed. Behind these unique performances, Intracellular Flow Cytometry require anyhow optimized immunoreagents for cell permeabilization, intracellular staining and fluorescent detection.

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