Mouse Embryonic Fibroblasts (MEFs) are critical biological reagents in stem cell research. Used as “feeder cells” to maintain Embryonic Stem (ES) cells and induced Pluripotent Stem (iPS), the quality of MEFs strongly influences Stem cell culture conditions, and may react differently depending on culture conditions. In parallel, MEFs can also be reprogrammed into a variety of more mature differentiated functional cell types (cardiomyocytes, neurons…). During daily discussions with Stem cell biologists, I have been able to collect Frequently Asked Questions and technical tips related to MEFs.
Traditionally, Fetal Bovine Serum (FBS) has been used as an additive to cell culture media for the in vitro growth of pluripotent stem cells. FBS is an inherently variable component of the cell culture system, so consistent performance demands extensive screening of manufactured lots. To circumvent this problem, companies began offering “stem cell qualified” serum. One issue with this solution is that the qualification is not always performed on a stem cell line relevant to the researcher’s own cell lines. Years later, researchers began using Life Technologies Knockout® Serum Replacement (KOSR) to grow and maintain undifferentiated human embryonic stem (ES) cells in culture. While KOSR has shown to be more stable than FBS and performs better in maintaining undifferentiated ES and induced pluripotent stem (iPS) cells, it is expensive and many researchers are today looking for a more cost effective media solution for their pluripotent stem cell culture. To address this need in the scientific community, GlobalStem now offers a more economical solution with their new PluriQ™ Serum Replacement. [Read more…]
Human Newborn Foreskin Fibroblasts (NuFF cells) are genuine feeder cells for Stem cell research. Mitotically inactive NuFF cells (by irradiation or Mitomycin C chemical treatment) can efficiently maintain healthy undifferentiated human pluripotent cell status and support cell reprogramming. In this post, you’ll find the answers to Frequently Asked Questions collected by tebu-bio’s cell biology experts regarding these feeder layers.
I hope these FAQs will help you in your Stem cell research! Have a good read.
In 2010, a method for integration-free reprogramming by transfecting modified mRNA reprogramming factors was published (1, 2). The use of mRNA to induce reprogramming of somatic cells overcomes the inherent problems incurred by introducing viral vectors and/or integrating DNA to target cells. mRNA reprogramming factors are titratable and controllable with regards to the ratios, concentrations, and timing of factor expression. The mRNA reprogramming system also poses none of the biosafety risks surrounding viral and DNA-based systems. [Read more…]