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tebu-bio's blog - Acting and reacting in life sciences and biotechnologies
  • Home
  • Research areas
    • ADME-Tox
    • Biomarkers
    • Cell Biology and Signalling
    • Cell Sourcing – Cell Culture Technologies
    • Drug Discovery
    • Gene Expression – Molecular Biology
    • Stem Cells
    • Supplying Discovery Tools
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  • Meet the authors
Supplying Discovery Tools

Immunoassays with collagen: Tips to optimize anti-collagen antibody performance

26/07/2018 by Isabelle Nobiron No Comments

Dr Yong Zhang, Ph.D. is a senior scientist and R&D Manager for antibody generation and assay development at Rockland Immunochemicals, Inc. who are recognised and renowned for their high quality primary antibodies, secondary antibodies and many other reagents for Life Sciences.

Dr Yong Zhang’s special focus is on collagen related products, and I’m sure you’ll find his expert tips and tricks that he kindly shares in this post very useful if you’re working with collagen immunoassays. Read on to the end, where you’ll find a selection of high quality, reliable collagen related reagents that may be very useful for your projects.

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Supplying Discovery Tools

Versatile colorimetric method to measure Hyaluronic acid (HA)

13/04/2016 by Ali El Baya, PhD No Comments
HA structure

Fig 1: Structure of HA

Hyaluronic acid (Hyaluronan) belongs to the group of glycosaminoglycans (GAGs), but unlike other GAGs like Heparan sulfate, Chondroitin sulfate or Keratan sulfate, it cannot be found as a proteoglycan. It represents a non-sulfated polysaccharide consisting of alternating residues of D-glucuronic acid and N-acetylglucosamine (Fig 1). In the human umbilical cord and synovial fluid, the size of HA is reported to be about 3,000,000 Da (a flexible chain of 4000 disaccharide units).

HA is one of the major components of the extracellular matrix and it absorbs high amounts of water thus giving tissues the ability to resist compression. Furthermore, Hyaluronan contributes significantly to cell proliferation and cell migration. As HA levels often correlate with malignancy and poor prognosis of certain cancers, it can be used as a tumor marker. As Hyaluronan plays a role in skin healing, it’s a widely used ingredient of skin-care products.

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Supplying Discovery Tools

3 easy colorimetric assays to measure ECM components

05/11/2015 by Ali El Baya, PhD 4 Comments
Sircoll for soluble and insoluble collagen detection - flow chart

Fig.1: Collagen source and Sircol assay choice.

Matrix components are key R&D elements in pharmaceutical and cosmetology with tremendous impacts on tissue regeneration, skin repair, wound healing, allergy and inflammation, surgery, ageing diseases…

Collagen, Elastin, and Proteoglycans are major components of the Extracellular Matrix (ECM) where they form highly complex networks. ECM builds up an environment which  supports  surrounding cells and plays a crucial role in cell-to-cell communication, cell adhesion, and differentiation.

The monitoring of ECM components during experimental approaches requires robust and user-friendly in vitro assays. In the past experimental methods (e.g. soluble collagen measurement) were laborious and based on the use of harmful chemicals. Today, easy-to-use and straightforward methods with colorimetric read-outs are used daily.

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Biomarkers

Extracellular matrix in cardiovascular diseases

09/09/2015 by Ana Arraztio No Comments

Extracellular matrix (ECM) was previously considered as some kind of ‘glue’, sticking cells together to form tissues, but is now a major area of research in a number of diseases, including Cardiovascular Diseases (CVDs).

ECM is a dynamic biomaterial that provides strength and elasticity, as well as anchor points of interactions with cell surface receptors, and availability of growth factors. Proper formation and organisation of the ECM is essential for cell and tissue homeostasis (1).

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