Believe it or not, some 20 years ago, performing an ELISA would take around 3 days. Now we are used to ELISAs that can be performed in one day, or even before lunch! (OK, sometimes before Spanish lunch times, which usually are at 14:00 or 15:00).
Fast ELISAs are a new step forward. By performing one washing step only, and through optimised protocols, results can be achieved in around 2.5 hours (this would really be before usual European lunch times… or mid-morning Spanish coffee)!