There are many, many ELISA tests out there to choose from, and making the best selection can sometimes be a bit daunting. In this post, let’s take a look at the key points to keep in mind.
At the recent meeting of all RayBiotech distributors, Isabelle Nobiron PhD (Product Specialist, tebu-bio), was proud to receive the 2017 Outstanding Distributor Award – Best in Europe.
During this meeting, which took place in Guangzhou (China), RayBiotech (Norcross, USA) also appointed tebu-bio as one of the 3 centers in the world to perform contract research for their high density arrays. Up to 1000 human proteins, 200 mouse and 67 rat biomarkers can be tested on semi-quantitative or quantitative proteomics microarray platforms.
tebu-bio has been a longstanding, recognised RayBiotech Certified Services provider, performing outsourced immunoassays at their European Headquarters situated near Paris (full range of services tebu-bio services here).
RayBiotech, Inc. develop cutting edge proteomics tools for the advancement of basic and clinical research, and the facilitation of drug and biomarker discovery. Their product-line covers 2,000+ rigorously tested ELISA tests (covered by a 90-day 100% satisfaction guarantee), Multiplex ELISA Arrays (Quantibody®), Membrane- or glass-slide Antibody Arrays (C-Series, G-Series, L-Series, E-Series), Functional assays, Research antibodies and Recombinant proteins.
tebu-bio provides their broad range of products to researchers across Europe. For more details, contact your local tebu-bio office.
RayBiotech manufactures over 2 600 ISO certified and rigorously tested ELISA tests, all backed by a 100% guarantee. All RayBio® ELISAs are manufactured in the USA and validated for exceptional reproducibility.
Every kit is subjected to a battery of rigorous product-specific quality control tests and manufactured in compliance with GMP and ISO 13485 standards.
Although the Sandwich ELISA format is the most commonly used, other immunoassay formats are also available to allow for a complete tool box, from phosphorylation studies to transcription factor activity assays.
In this post, I’ll be taking a look at the 6 formats available.
Note: up to December 31st, you can get 15% discount off these ELISA test kits. [Read more…]
Expression systems – removing their contaminants
CHO, E. Coli and Pichia are useful expression systems for drug production. This can be for proteins, enzymes or antibody productions. Research and development for therapeutic perspectives requires a high level of quality controls. Such expression systems contain +1000 host cell proteins (HCPs). A major challenge in biological component production is to remove this HCP contamination. In order to monitor removal efficiency (during protein purification, as mentioned further on in this article), Canopy Biosciences have developed and optimised HCP detection kits based on the principle of the sandwich ELISA test as illustrated below.
If your research is related to obesity and adipose tissue, you may be interested by the selection I have performed below of immunoassays (including ELISA tests and antibody arrays), as well as primary cell models related to this topic.
Obesity and the diseases arising from it (diabetes, cardiovascular disease and cancer) constitute a major health challenge to the industrialized world. In the past decade, it has become apparent that adipose tissue is a major endocrine organ which secretes numerous soluble factors and regulates many processes related to glucose homeostasis, lipid and protein metabolism, inflammatory and immune responses… [Read more…]
Many ELISA kits – one analysis tool
Analyte quantification (in pg/ml or ng/ml) such as IL-6, TNFalpha and numerous other proteins is conveniently performed with immunoassays called ELISA kits. At tebu-bio, we not only provide more than 10 000 ELISA kits but we also support researchers through our techserv team who are on-hand to suggest the best kits and to answer any questions.
Being part of that team, I have noticed a number of often asked questions that can be resumed in one : What is the best method for data analysis? [Read more…]
The mitogen-activated protein kinase (MAPK) signaling pathway is activated by a number of extra and intracellular stimuli including cytokines, growth factors, and hormones as well as stressors such as oxidative and ER stress. This pathways plays a key role in the regulation of many cellular processes including proliferation, differentiation, the stress response, motility, growth, differentiation, survival, and death. Abnormal MAPK signaling may contribute to increased or uncontrolled cell proliferation and/or resistance to apoptosis. To study this complex pathway, several tools are available, from the pathway specific arrays for an initial screen, to phospho-specific ELISA tests for individual target validation. This post aims at helping you to easily identify tools to explore this pathway in your samples (from arrays to phospho-ELISAs). However, I could not start without showing you once more one of these eye-catching illustrations of cell signalling pathways. I’ll let you explore it to dig out the MAPK protein cascade among all of them (a kind of Where’s Wally for the researcher !).
With more than 170,000 publications in 2016, inflammation remains a “hot” topic in today’s Life Science research (source: Google Scholar). Interestingly, the ELISA test is still one of the most popular tools for the accurate quantification of human cytokines involved in this biological process. Here, we review the 5 protein targets most studied by our clients, together with their favourite ELISA kits by RayBiotech in the field of inflammation.