Cell-Based ELISA tests now enable precise measurement of the phosphorylation status or expression profiles of target proteins (biomarkers) in whole cells. In this post, a review of high performing cell-based assays is made.
Nipah Virus Infection (NiV) is an emerging infectious disease of public health importance in the South-East Asia Region (source: World Health Organization). Along with Hendra virus (HeV), NiV is part of a new genus designated Henipavirus. Fruit bats have been identified as the natural reservoirs. Nevertheless, pigs, horses, goats, sheep, cats and dogs can also be infected and transmit the virus.
Symptoms of NiV infection are similar to influenza and in some cases can lead to encephalitis. The case fatality rate is high (40-70%) and there is no specific treatment. While able to bind with cellular EphrinB2, NiV G Protein (NiV-G) exhibits the antigenic epitopes and conformation necessary for specific antigen–antibody recognition. For this reason, Meridian Life Science have recently developed new Nipah virus glycoprotein (NiV-G) products suitable for the R&D projects of NiV using ELISA tests. [Read more…]
Hyaluronic acid (also called HA or Hyaluronan) is a glycosaminoglycan with elevated viscosity, enabling tissues (eye, skin, joint and synovial fluid…) to resist to physical and mechanical constraints (torsion, flexion…). Over time, when HA production declines, tissues progressively lose these tensile properties, leading to wrinkles and fold, weak re-epithelisation and age-related troubles. But HA is also involved in many other chronic and cancer-related diseases. In this post, we’ll review one of the most popular HA quantification assays (ELISA test), known to be highly sensitive and robust, appreciated by researchers involved in cosmetology and drug discovery. [Read more…]
PBL assay specializes in the development of IFN related research Tools. One of their most appreciated assay kit by the IFN research community is the ELISA test kit for all Human IFN Alpha subtypes (41115).
Its unique features such as high sensitivity, matrix compatibility and broad detection of IFN alpha subtypes explain this popularity. [Read more…]
RayBiotech has just announced the release of a new ELISA test technology breakthrough that I am quite thrilled to share with you. If you have precious samples with low abundant target and which require high sensitivity, then this post will be of interest to you!
The RayBio® Immuno-Quantitative ELISA (IQELISA) kit is an innovative assay platform that combines the specificity and ease of use of a sandwich ELISA test with the sensitivity of real-time PCR. This results in an assay that is cutting edge and yet familiar, enabling the use of only 1/10th of the typical sample volume while providing up to 10x more sensitivity than a traditional ELISA test. The IQELISA technology is completely novel, patent pending, and has no equivalent in the market at this time. [Read more…]
mRNA vaccines are developing very quickly. There are already 25 ongoing clinical trials against cancers, as well as clinical trials against infectious diseases such as HIV. Recombinant monoclonal antibody (mAB) based treatments that have lead to the most successful therapies could even switch to mRNA coding heavy and light chains for direct antibody production into the patient (as of yet still at the level of mouse models).
Indeed, messenger RNAs (mRNA) are now new biological entities to take in consideration for the development of efficient and personalized therapies. They have several advantages over conventional drugs: [Read more…]
There are many, many ELISA tests out there to choose from, and making the best selection can sometimes be a bit daunting. In this post, let’s take a look at the key points to keep in mind.
At the recent meeting of all RayBiotech distributors, Isabelle Nobiron PhD (Product Specialist, tebu-bio), was proud to receive the 2017 Outstanding Distributor Award – Best in Europe.
During this meeting, which took place in Guangzhou (China), RayBiotech (Norcross, USA) also appointed tebu-bio as one of the 3 centers in the world to perform contract research for their high density arrays. Up to 1000 human proteins, 200 mouse and 67 rat biomarkers can be tested on semi-quantitative or quantitative proteomics microarray platforms.
tebu-bio has been a longstanding, recognised RayBiotech Certified Services provider, performing outsourced immunoassays at their European Headquarters situated near Paris (full range of services tebu-bio services here).
RayBiotech, Inc. develop cutting edge proteomics tools for the advancement of basic and clinical research, and the facilitation of drug and biomarker discovery. Their product-line covers 2,000+ rigorously tested ELISA tests (covered by a 90-day 100% satisfaction guarantee), Multiplex ELISA Arrays (Quantibody®), Membrane- or glass-slide Antibody Arrays (C-Series, G-Series, L-Series, E-Series), Functional assays, Research antibodies and Recombinant proteins.
tebu-bio provides their broad range of products to researchers across Europe. For more details, contact your local tebu-bio office.
Having access to highly reproducible and fully validated ELISA tests is crucial in today’s Life Science research. Although the Sandwich ELISA format is the most commonly used, other immunoassay formats are also available to allow for a complete tool box, from phosphorylation studies to transcription factor activity assays.
In this post, I’ll be taking a look at the 6 formats available. [Read more…]
Expression systems – removing their contaminants
CHO, E. Coli and Pichia are useful expression systems for drug production. This can be for proteins, enzymes or antibody productions. Research and development for therapeutic perspectives requires a high level of quality controls. Such expression systems contain +1000 host cell proteins (HCPs). A major challenge in biological component production is to remove this HCP contamination. In order to monitor removal efficiency (during protein purification, as mentioned further on in this article), Canopy Biosciences have developed and optimised HCP detection kits based on the principle of the sandwich ELISA test as illustrated below.