• Home
  • Research areas
    • ADME-Tox
    • Biomarkers
    • Cell Biology and Signalling
    • Cell Sourcing – Cell Culture Technologies
    • Drug Discovery
    • Gene Expression – Molecular Biology
    • Stem Cells
    • Supplying Discovery Tools
  • Contact us
  • Meet the authors
  • Facebook
  • LinkedIn
  • Twitter
  • YouTube
Home
Research areas
    ADME-Tox
    Biomarkers
    Cell Biology and Signalling
    Cell Sourcing - Cell Culture Technologies
    Drug Discovery
    Gene Expression - Molecular Biology
    Stem Cells
    Supplying Discovery Tools
Contact us
Meet the authors
tebu-bio's blog - Acting and reacting in life sciences and biotechnologies
  • Home
  • Research areas
    • ADME-Tox
    • Biomarkers
    • Cell Biology and Signalling
    • Cell Sourcing – Cell Culture Technologies
    • Drug Discovery
    • Gene Expression – Molecular Biology
    • Stem Cells
    • Supplying Discovery Tools
  • Contact us
  • Meet the authors
Supplying Discovery Tools

Tropical & vector-borne disease antibodies : find your match for Rapid Assays!

17/04/2019 by Isabelle Nobiron, PhD No Comments

Tropical and vector-borne diseases account for more than 17% of all infectious diseases, causing more than 1 million deaths annually. Recognizing infected patients early in their clinical course is critical to researchers working in developing tools to prevent outbreaks. Meridian offer a range of antigens and antibodies suitable for the development of sensitive and specific immunoassays that detect tropical and vector-borne diseases. Their antibody pairs have already been validated in various immunoassays. You won’t waste time finding the right match, it has already been validated for you.

In the first part of this post, I’d like to introduce a selection of ready-to-use antibody pairs available for the main tropical and vector-borne diseases such as Chikungunya, Dengue, Ebola, Leishmania, Malaria, Yellow fever and Zika to name but a few. I’ll focus in the second part on Flavivirus immunoassay development with troubleshooting and usage tips coming from Meridian scientists to help overcoming high epitope cross-reactivity, a common issue in this specific virus family.

Continue reading
News

Thiostrepton: a novel lead compound against Dengue?

10/06/2015 by Philippe Fixe, PhD No Comments
Thiostrepton Focus Biomolecules tebu-bio Catalog # 10-2108
Dengue Virus (DenV) is transmitted by mosquito vectors. It infects 50-100 million people each year and is at the origin of Dengue Fever and the more lethal Dengue Hemorrhagic Fever (DHF) and Shock Syndrome (DSS) leading to an estimated 500,000 cases of DHF and 22 000 deaths. The World Health Organization (WHO) estimates that 40% of the world’s population is at risk of infection. In the June issue of Journal of Biomolecular Screening, investigators at San Diego State University (Dept of Biology) and Institute Pasteur Korea (Seoul, South Korea) developed a multiplexed cell-based assay for the identification of modulators of pre-membrane processing as a target for the discovery of DenV inhibitors. (1) The DenV pre-membrane protein (prM) is an essential chaperone for the viral envelope protein which prevents premature fusion with vesicles during viral export. Inhibition of pre-membrane protein cleavage restricts fusion and represents, thus, a novel druggable target. The new in vitro assay developed in this study, is the first described cell-based assay that monitors DenVprM processing within the classical secretory pathway. In a pilot screen of 1,280 small molecules on that assay, Thiostrepton, a known cyclopeptide Antibiotic and FOXM1 inhibitor,  was identified as a novel positive hit in this assay (IC50=4.94 µM). The utility of this novel assay has been proven by the identification of Thiostrepton (available at Focus Biomolecules cat. nr 10-2108) which may be a novel lead compound for the discovery of new drugs effective against Dengue Virus.
Thiostrepton Focus Biomolecules tebu-bio Catalog # 10-2108

Thiostrepton – Antibiotic and FOXM1 inhibitor. C72H85N19O18S5 – CAS No: 1393-48-2, 98% by TLC and HPLC at Focus Biomolecules.

  1- Stolp Z.D. et al. “A Multiplexed Cell-Based Assay for the Identification of Modulators of Pre-Membrane Processing as a Target against Dengue Virus” (2015) J. Biomol. Screen. 20:616-626. DOI: 10.1177/1087057115571247. Related items A selection of Abnova’s primary antibodies and ELISA kits in relation to Dengue Fever is available here. A few examples:  
Dengue Fever Cat. Nr Product Name Application
Antibody for Dengue Virus 157MAB4043 Dengue virus Types 1,2,3,4 monoclonal antibody, clone BDI419 Dot,IF,EIA
Antibodies for Envelope Protein 157MAB8901 Dengue virus E-D3 monoclonal antibody, clone 5j122 ELISA,WB-Re
Antibodies for NS1 protein 157MAB13532 Dengue virus NS1 monoclonal antibody, clone HM026 ELISA,IF,LFIA
   
Subscribe to e-Newsletters on your favourite topicsInterested in learning more about tools like this? Subscribe to thematic newsletters on your favourite research topics.
 
tebu-bio

LAB SERVICES OUTSOURCING

• Protein production

• Plasmid Preps

• 3D Cell Culture
LEARN MORE

Most popular posts

  • HeLa cells: Origin of this important cell line in life science research
  • From RUO to IVD - the acronym guide to reagents' intended use
  • How to choose the perfect buffer to get a pure, stabilised, functional protein
My Tweets

Top contributors

Dimitri Szymczak, PhD
Dimitri Szymczak, PhD
  • How can you get your mRNA ready in only a few weeks?
  • CleanCap: The new capping standard for self-amplifying mRNA
Frédéric Samazan
Frédéric Samazan
  • Immuno-Oncology: 6 must-haves to boost your projects
  • MagIC Beads – The future of RNA capture kits
Isabelle Nobiron, PhD
Isabelle Nobiron, PhD
  • COVID-19: relevant cell culture models
  • Immunoassay development: reliable sources of quality reagents
Isabelle Topin, PhD
Isabelle Topin, PhD
  • Antibody Purification: Tips and Tricks
  • Monoclonal antibodies – all you need to know about antibody generation
Philippe Fixe, PhD
Philippe Fixe, PhD
  • How to monitor inflammatory cytokines in anti-COVID drug discovery
  • New SARS-CoV/ACE2 inhibitor assays & small molecules for drug discovery

Privacy & Cookies: This site uses cookies. By continuing to use this website, you agree to their use.
To find out more, including how to control cookies, see here: Cookie Policy

Copyright © 2018 - tebu-bio - visit our main website at tebu-bio.com