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    ADME-Tox
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Tebubio's blog - Acting and reacting in life sciences and biotechnologies
  • Home
  • Research areas
    • ADME-Tox
    • Biomarkers
    • Cell Biology and Signalling
    • Cell Sourcing – Cell Culture Technologies
    • Drug Discovery
    • Gene Expression – Molecular Biology
    • Stem Cells
    • Supplying Discovery Tools
  • Contact us
  • Meet the authors
Supplying Discovery Tools

New – you know CRISPR, now it’s Prime Editing

28/10/2019 by Dimitri Szymczak, PhD No Comments

This month, a new leap forward in gene editing has been published in Nature under the title ‘Search-and-replace genome editing without double-strand breaks or donor DNA’. DOI: 10.1038/s41586-019-1711-4. Let’s take a look at prime editing to get a clear overview.

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Supplying Discovery Tools

HIT-CAS9: How to fully control gene editing

18/06/2019 by Dimitri Szymczak, PhD No Comments

Are you looking for a simple means to boost the efficiency of gene editing and also to reduce the off-target? Yes, it does exist – in one word: 4-hydroxy Tamoxifen. That’s the HIT-CAS9.

Hybrid drug inducible CRISPR/Cas9 technology, HIT-Cas9

Zhao et al. revealed tight and effective drug control of the CRISPR Cas9 based on 4-Hydroxytamoxifen.

They built a fusion between a mutated human estrogen receptor (ERT2) and the Cas9 endonuclease, which makes the CRISPR Cas9 dependent on 4-hydroxytamoxifen.

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Supplying Discovery Tools

Easy single-cell isolation with Smart Aliquotor

03/07/2018 by Dimitri Szymczak, PhD No Comments
Smart Aliquotor

Single-cell isolation allows genomic and transcriptomic analysis of one individual cell. It is also required to build a monoclonal cell line from rare cell isolation, that could be for example CRISPR-CAS9 gene edited cells. There are 3 popular methods: serial dilution, micro-manipulation and flow cytometry. None of these are easy or simple, and they often require expertise and experience. Fortunately, a new solution for everybody has come! It’s designed to isolate a single cell in a few seconds, and it’s (quite appropriately) called the Smart Aliquotor.

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Gene Expression - Molecular Biology

Peace of mind with CRISPR-CAS9 gene editing

24/05/2018 by Dimitri Szymczak, PhD 2 Comments

CRISPR-CAS9 is a powerful technology for gene editing. It allows targeted modifications into the genome of the cell lines, IPSCs and ESCs. Despite the principle being pretty simple, in practice its use requires some expertise, and it can even turn into a time consuming adventure… That’s why the best way to benefit from it is most certainly through affordable services.

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