Cosmetology

Open innovation in cosmetology at Cosmetic 360 – Paris, Oct. 13-14

30/09/2016 By Sara Pierre Leave a Comment

This year, the Cosmetic-360 Meeting organised by the world’s leading perfumery cosmetics network, Cosmetic Valley, will take place in Paris on October 13th & 14th.

As a member of this network, tebu-bio will again be participating in this trade show focussed on open innovations. This congress is an excellent opportunity to discover the innovative solutions designed by tebu-bio laboratories in the field of R&D and in vitro testings aiming at better understanding the mechanisms of action and further assessing the efficiency of ingredients.
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Oxygen and miRNAs in Cosmetology and Dermatology

25/08/2015 By Ana Arraztio Leave a Comment

A recent review by Nadim et al. casts some light on a Cosmetology and Dermatology, where circulating biomarkers, though studied to a certain extent so far, are yet unknown for many skin models. (1) A first element to have in mind when considering the skin-related experimental model is the “Oxygen level”. Oxygen levels may contribute to different findings, and the in vitro models used so far may not be so physiologically relevant as initially thought.

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Anti cellulite compound evaluation with in vitro adipocyte-assays

01/07/2015 By Frédéric Dubor, PhD 1 Comment

Mechanisms leading to cellulite formation is complex. It involves lipid regulatory pathways and proinflammatory cross-talk that represent promising molecular targets in cosmetology. This post introduces a clever� in vitro adipocyte-based assay targeting adipocytokines to better determine the anti cellulite effects of cosmetics compounds.

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Hyaluronic acid – size matters!

13/01/2015 By Ana Arraztio 1 Comment

Hyaluronan (HA) Competitive ELISA standard curve (cat. nr 117K-1200-1ea)

Hyaluronic acid (HA), or hyaluronan, is an ubiquitous, very high molecular mass polysaccharide that has applications in a variety of fields, including cosmetics, some types of surgery (e.g. opthalmic) and regenerative medicine. It can even be present as a contaminant in some bio-production processes. HA has also been suggested as a possible biomarker for Alzheimer’s disease (AD).

HA acts as a molecular shock-absorber and stabilizer for cells. Its visco-elastic properties, biologically speaking, are valuable for separating tissue and maintaining shape. It is a key in tissue lubrication, and it may play a role in wound repair. It is the ideal choice for some implants, as it does not usually cause an immune response (contrary to what may happen with some biomaterials). Size of the HA used in therapy has an impact on its success. Usually, higher weight forms usually render longer benefits. For bio-production, however, smaller HA forms are usually the main concern.

So, depending on the reason why you are studying this marker, keep size in mind in order to choose the best assay to measure the HA levels in your experimental model. It is of key importance that whatever product you use, analysis of the HA sizes detected with it are clearly mentioned in the technical documentation.

Hyaluronic acid (HA) sandwich ELISA standard curve (direct assay cat. nr 117K-4800-1kit)

A post by my colleague Dr. Philippe Fixe is of great help for choosing the right assay!

It’s not that these assays will allow you to discriminate between high- and low-weight HA. What is intended here is that these assays will allow you to detect all or part of the HA forms depending on its weight. And that may be crucial, as you may be using the assay that does not detect the HA sizes relevant for your experiment.

It is also possible now to outsource HA measurements to an external lab performing regularly validated Hyaluronic acid-specific immuno-assays. As an example, Echelon’s Competitive or Sandwich HA ELISAs outsourcing by tebu-bio’s lab in cosmetology or drug discovery.

Any comments? Feel free to share them below!

MDR1 activity enhancement reduces senescence markers

05/01/2015 By Philippe Fixe, PhD Leave a Comment

During the last International Federation of Societies of Cosmetic Chemists (IFSCC) congress (Paris, Oct. 2014), Hajem N. et al (ALES Groupe) have shown that by enhancing MDR activity of fibroblasts grown� in vitro, a positive effect on fibroblast cells was detected. In addition to directly testing MDR1 activity, the authors also used a genomic profiling approach to correlate the protective effect of their D-GlyOx complex with a reduction in senescence biomarkers on treated fibroblasts.

tebu-bio’s laboratories supported the Ales Groupe (LIERAC, PHYTO, CARON, Laboratoire DUCASTEL) by providing fibroblast cellular models and measuring the anti-ageing effect of their D-GlyOx complex by genomic profiling,

Source:� Hajem N. et al “MultiDrug Resistance (MDR) proteins: active protective system, source of cell longevity” – International Federation of Societies of Cosmetic Chemists (IFSCC) congress – Oct. 27-30, 2014 – Paris) Poster # P-080.

Sircol for soluble and insoluble collagen assays!

23/09/2014 By Ali El Baya, PhD 7 Comments

Measuring soluble collagen in biological samples is a straightforward experiment which can be done with the Sircol soluble collagen assay using Sirius Red dye (Sircol dye) in a convenient manner (we explored this method in a recent post). Nevertheless, up to now it has been a challenge to measure insoluble collagen.

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Primary cell sourcing: Cryopreserved Human Adult Sebocytes

13/06/2014 By Philippe Fixe, PhD Leave a Comment

Sebocytes are emerging cellular models to study skin biology but also to design new compounds to regulate acne, seborrhoea and other sebaceous gland-related diseases. Easy access to cryopreserved and well-qualified sources of Human Adult Sebocytes will boost skin research and compound testing projects.

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How to select Hyaluronic Acid (HA) quantitative tests?

02/06/2014 By Philippe Fixe, PhD 4 Comments

Hyaluronic acid (also called HA or Hyaluronan) is a glycosaminoglycan with unique characteristics. HA possesses elevated viscosity, enabling tissues (eye, skin, joint and synovial fluid…) to resist to physical and mechanical constraints (torsion, flexion…). Over time, when HA production is declining, tissues progressively loss these tensile properties, leading to wrinkles and fold, weak reepithelization� and age-related troubles. [Read more…]

1 simple way to rapidly quantify Collagen: Sircol Collagen Assay

16/05/2014 By Philippe Fixe, PhD 2 Comments

Collagen is a structural protein expressed in mammalian tissues (ex. skin, muscles, tendons, ligaments, cartilages, blood vessels…). Together with Elastin and Keratin, Collagen fibres give� connective properties to mammalial tissues ensuring their physiological form but also firmness, strength and flexibility.� Needless to say, Collagen is trendy… a simple search in Google using the term “Collagen” clearly shows the economical dimension of this protein in well-being.

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