If you work in Biology, you’ve most certainly heard of HeLa cells, as they have been around for over 60 years and are some of the most extensively used cell lines in Biomedical research. But where did these cell lines come from? [Read more…]
Functionality and viability of primary cells can be impaired by incorrect thawing procedures, storage or culture conditions. It’s generally admitted that applying the same protocols as for cell lines leads will lead to bad cell quality. Well, based on our experience with primary cells, here are a few tips you can follow to ensure you get the best performance.
Human Peripheral Blood Mononuclear Cells (hPBMCs) are essential for designing cellular models to be used in cell therapy and drug discovery research programs [1-7, 12]. Being able to access reliable and ethical sources of well-characterized hPBMCs is now becoming fundamental for running physiologically relevant cell-based assays. A good opportunity to discuss the expanding applications for hPBMCs in drug development, that Patricia Bresnahan, PhD (Global Marketing Director at HemaCare Corporation) has observed over the last years.
Understanding how mammalian cells function requires a dynamic perspective. Recent improvements in our abilities to perform fluorescence microscopy on primary cells, coupled with advances in pipelines for quantifying and extracting data, have made possible a better understanding of the temporal complexity of cell signalling pathway. Due to the heterogeneity seen in both eukaryotic and prokaryotic cell populations, study at the single cell resolution with living cells is currently the best solution to understand the dynamics between environmental conditions and cellular behaviour.
However as living cells don’t stay still, classical imaging and studying methods present some drawbacks for single cell analysis and tracking such as: [Read more…]
The islets of Langerhans are the regions of the pancreas that contain its endocrine (i.e., hormone-producing) cells. Discovered in 1869 by German pathological anatomist Paul Langerhans, the islets of Langerhans constitute approximately 1% to 2% of the mass of the pancreas. There are about one million islets distributed throughout the pancreas of a healthy adult human. Each is separated from the surrounding pancreatic tissue by a thin fibrous connective tissue capsule. The islets of Langerhans contain beta cells, which secrete insulin, and play a significant role in diabetes.
Islets are widely used for transplantation to restore beta cell function from diabetes, offering an alternative to a complete pancreas transplantation or an artificial pancreas. Because the beta cells in the islets of Langerhans are selectively destroyed by an autoimmune process in type 1 diabetes, islet transplantation is a means of restoring physiological beta cell function in patients with type 1 diabetes.
Human Islets for Research (HIR)® are primary human islets processed from organ donor pancreases that have been approved for research but not for clinical transplantation of either the pancreas or the isolated islets. HIR® are obtained in a proprietary process of pancreas digestion and islet purification that results in uniformly high quality HIR® for delivery to diabetes investigators. Quality Control (QC) testing is routinely performed prior to release to assure uniform quality and function of these islets available for research. [Read more…]
I have to admit that I never received the Fields Medal in Mathematics. Therefore, I won’t be able to develop this equation and prove that I’m right. However, what I can prove, is that in cell culture, 2 = 5. How is this possible?
Complex cell culture systems are emerging as key tools to improve physiological relevance of in vitro assay systems. There have been two main ways by which investigators attempt to improve mimicking of physiological conditions in cell and tissue culture. The first is to develop more complex model systems where two or more cell types are co-cultured in a 3D structure either separated by membranes or in spheroids . The second is to incorporate fluidic-flow where the motion of the media itself has been shown to improve metabolic function and lifespan [2,3].
Despite the success of better recapitulating function at the cellular level using these two methods, neither of these approaches addresses the issue of the non-linear nature of the drug or toxicant exposure as is observed in an in vivo system . As a result, the capacity to accurately predict in vivo pharmacokinetics and pharmacodynamics still falls short reaching at best 60-70% accuracy [5,6].
So what’s new in this area? We were interested to discover the following system, which we think will be of interest to many researchers. Let’s take a closer look at the characteristics and how it can be used. [Read more…]
Thousands of researchers spend time pampering their primary cells and cell lines every day, with the aim of defining their optimal cell culture conditions. Researchers are in effect trying to control numerous cellular parameters (oxygene levels, fresh media supply, incubation temperature…) and monitor their “viability” on a regular basis. But what about the internal cellular temperature? Have you ever considered this biological parameter to monitor the “wellness” of your cultivated cells? In this post, let’s take a look at the characteristics of a new, cell permeable, fluorescent thermometer for living cells.
In a previous post, I introduced several models to study the cardiac human system. This first post introduced human aortic, brachiocephalic, carotid artery and coronary artery cells isolated by Cell Applications Inc. Here is the second part of this inventory of cellular models to study the human cardiac system, where I’ll be highlighting human internal thoracic artery, pulmonary artery, subclavian artery cells, cardiac fibroblasts and cardiomyocytes.
Later on, I’ll conclude this series by part III, referring to animal cellular models for studying the cardiac system. But let’s now concentrate on today’s topic! [Read more…]
In this post, I’d like to introduce human aortic, brachiocephalic, carotid artery and coronary artery cells isolated by Cell Applications Inc. In a future post, I’ll be highlighting human internal thoracic artery, pulmonary artery, subclavian artery cells and cardiomyocytes (now published here).
After taking a look at several cell types as models for studying different aspects of cardiovascular functions and diseases, I’ll cover some recently published results highlighting the importance of securing your primary cells sourcing.