• Home
  • Research areas
    • ADME-Tox
    • Biomarkers
    • Cell Biology and Signalling
    • Cell Sourcing – Cell Culture Technologies
    • Drug Discovery
    • Gene Expression – Molecular Biology
    • Stem Cells
    • Supplying Discovery Tools
  • Contact us
  • Meet the authors
  • Facebook
  • LinkedIn
  • Twitter
  • Vimeo
Home
Research areas
    ADME-Tox
    Biomarkers
    Cell Biology and Signalling
    Cell Sourcing - Cell Culture Technologies
    Drug Discovery
    Gene Expression - Molecular Biology
    Stem Cells
    Supplying Discovery Tools
Contact us
Meet the authors
Tebubio's blog - Acting and reacting in life sciences and biotechnologies
  • Home
  • Research areas
    • ADME-Tox
    • Biomarkers
    • Cell Biology and Signalling
    • Cell Sourcing – Cell Culture Technologies
    • Drug Discovery
    • Gene Expression – Molecular Biology
    • Stem Cells
    • Supplying Discovery Tools
  • Contact us
  • Meet the authors
Supplying Discovery Tools

New – you know CRISPR, now it’s Prime Editing

28/10/2019 by Dimitri Szymczak, PhD No Comments

This month, a new leap forward in gene editing has been published in Nature under the title ‘Search-and-replace genome editing without double-strand breaks or donor DNA’. DOI: 10.1038/s41586-019-1711-4. Let’s take a look at prime editing to get a clear overview.

Continue reading
Supplying Discovery Tools

Gene knock-out in hard-to-transfect cells

22/10/2019 by Dimitri Szymczak, PhD No Comments

Gene knock-out, which is gene editing leading to loss of function, just requires delivering into cells the 2 CRISPR system actors: the CAS9 endonuclease and the specific guide RNA to target the gene of interest. Thus, simple transfection of CleanCap CAS9 mRNA and gRNA followed by cell isolation with the smart aliquotor is a convenient and robust method. Howevery, what can you do if the cells are hard-to-transfect?

Continue reading
Gene Expression - Molecular Biology

Optimizing the CAS9 messenger RNA for vector-free gene editing

28/06/2017 by Dimitri Szymczak, PhD No Comments

Sequence engineering and chemical modification of CAS9 mRNA

Cas9 Western Blot in CD34+ cells - Trilink poster "“Maximizing Translation of Cas9 mRNA Therapeutics by Sequence Engineering and Chemical Modification”"

Extract from poster “Maximizing Translation of Cas9 mRNA Therapeutics by Sequence Engineering and Chemical Modification”.

For transgenesis and therapeutic perspectives, vector-free delivery of the CAS9 for gene editing is highly preferable. It avoids the risk of hitting the genome due to the vector integration. Directly using CAS9 mRNA is a top option.

Furthermore, as a new promising class of therapeutic biologics, messenger RNA are the subject of numerous studies.

This year, Matthew Porteus’s and Trilink’s teams revealed key and practical results at the ASGC with a poster you can download below.

Notably, there is a large screen of the mRNA CAS9 modifications based on the indel formation. This interesting comparison includes also CAS9 RNP and reveals the high performance of the CleanCap U-depleted 5-moU CAS9 mRNA. We can also note that for the CleanCap CAS9 mRNA HPLC purification is not required.

“Maximizing Translation of Cas9 mRNA Therapeutics by Sequence Engineering and Chemical Modification”
Download your copy of the poster here.

 


Subscribe to e-Newsletters on your favourite topics

Interested in learning more about tools like this?

Subscribe to thematic newsletters on your favourite research topics. 
Gene Expression - Molecular Biology

Cas9 mRNA optimized for genome editing

07/09/2015 by Philippe Fixe, PhD 1 Comment
CRISPRCas9 genome editing complex

CRISPR/Cas9 is relatively simple to implement, as the researcher fully controls the experimental design of the tools, from the sgRNA sequence to the Cas9 protein.

Let’s quickly look back to the basics… CRISPR Associated Protein 9 (Cas9) functions as part of the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) genome editing system.
In this system, a single guide RNA (sgRNA) sequence targets a site of interest in the genome while the Cas9 protein performs a double stranded DNA cleavage at the targeted genomic location. CRISPR/Cas9 system can be widely used to express or silence a gene, create mutations and variants… into the genome of the host cell.
Continue reading

Most popular posts

  • HeLa cells: Origin of this important cell line in life science research
  • Monoclonal antibodies - all you need to know about antibody generation
  • How to choose the perfect buffer to get a pure, stabilised, functional protein
My Tweets

Privacy & Cookies: This site uses cookies. By continuing to use this website, you agree to their use.
To find out more, including how to control cookies, see here: Cookie Policy

Copyright © 2018 - tebu-bio - visit our main website at tebu-bio.com

 

Loading Comments...