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    ADME-Tox
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Tebubio's blog - Acting and reacting in life sciences and biotechnologies
  • Home
  • Research areas
    • ADME-Tox
    • Biomarkers
    • Cell Biology and Signalling
    • Cell Sourcing – Cell Culture Technologies
    • Drug Discovery
    • Gene Expression – Molecular Biology
    • Stem Cells
    • Supplying Discovery Tools
  • Contact us
  • Meet the authors
Supplying Discovery Tools

Live cell imaging: the art of looking at biological processes in real time

06/07/2016 by Ali El Baya, PhD No Comments

Visualizing fixed cells and tissues only gives snapshots of cellular processes. To have a better insight into dynamic events ocurring in the cells or to vizualize interactions between various cellular components in real time (e.g., proteins, organelles, second messengers…), powerful microscopic approaches have been developed over the past decade. This post will review the recent live cell imaging probes developed by Goryo Chemicalsand available in Europe through tebu-bio.

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Cell Biology and Signalling

Have a closer look at GPCR mediated signaling!

02/06/2016 by Ali El Baya, PhD 2 Comments

Cell-based calcium assays have been a mainstay of GPCR (G protein coupled receptor) drug discovery and signal transduction research since the mid 1990s. However, a calcium readout alone might be ambiguous, because many cellular processes produce calcium. Furthermore, it does not differentiate between signalling pathways routed through the different types of heterotrimeric G proteins. While effects on GPCRs which exhibit effects on Gs proteins lead to an elevated level of the second messaeger cAMP by activating the activity of adenylate cyclase, signalling through Gi proteins inhibits adenylate cyclase and thus leads to a decrease of cAMP. Finally signalling through Gq proteins leads to phospolipase C activation which cleaves Phosphatidylinositol 4,5-bisphosphate (PIP2) to Diacetlyglycerol (DAG) and Inositol trisphosphate (IP3) which leads to an increase in Ca2+ level (for an overview see reference 1).

Dye based methods to measure Ca2+ are very well established, whereas there are no dyes available for most of the other second messengers in these signalling pathways.

Genetically encoded fluorescent biosensors for live cell discovery

That’s why Montana Molecular developed genetically encoded fluorescent biosensors for second messengers such as cAMP, DAG, PIP2, and Ca2+, references 2, 3).

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Drug Discovery

Intracellular Calcium measurement with Fluo-8

22/09/2015 by Ali El Baya, PhD 12 Comments

G Protein Coupled Receptors (GPCR) and ion channels are important targets in drug development. Both target classes represent membrane proteins which require convenient and robust cell-based assays for screening campaigns. Usually used as readout of such screenings, intracellular Calcium concentration implies the use of Ca2+ indicators able to meet demanding expectations of researchers engaged in assay development and screenings.

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