Today, I’d like to give some tips & tricks on how to select an antibody… by reading its datasheet.
Obvious as it may seem, the datasheet is in fact contractual. It states the characteristics and guaranteed performance of a given product in general, or an antibody in particular. So it should be read carefully before taking a purchasing decision. No matter if you saw a great antibody detecting protein X in polar bear in some publication. If polar bear is not one of the validated species in the datasheet, the antibody is not guaranteed and you won’t get a replacement or refund.
Believe it or not, some 20 years ago, performing an ELISA would take around 3 days. Now we are used to ELISAs that can be performed in one day, or even before lunch! (OK, sometimes before Spanish lunch times, which usually are at 14:00 or 15:00).
Fast ELISAs are a new step forward. By performing one washing step only, and through optimised protocols, results can be achieved in around 2.5 hours (this would really be before usual European lunch times… or mid-morning Spanish coffee)!
Immunoediting of cytokine signals provides another area of pathways which tumours utilise to evade and potentially escape from immunological targeting. This post aims at shedding some light into the Tumour Microenvironment (TME) and particularly on how the tumour recruits accomplices to grow and disseminate.