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Tebubio's blog - Acting and reacting in life sciences and biotechnologies
  • Home
  • Research areas
    • ADME-Tox
    • Biomarkers
    • Cell Biology and Signalling
    • Cell Sourcing – Cell Culture Technologies
    • Drug Discovery
    • Gene Expression – Molecular Biology
    • Stem Cells
    • Supplying Discovery Tools
  • Contact us
  • Meet the authors
Cell Biology and Signalling, Supplying Discovery Tools

How to activate or inhibit autophagy?

10/11/2016 by Ali El Baya, PhD No Comments

The term Autophagy was introduced by Christian de Duve during the Ciba Foundation Symposium on Lysosomes – which was held in London in February 1963. In 1974 he was honoured with the Nobel price in Physiology or Medicine for his pioneering research about peroxisomes and lysosomes. In 2016, once more, a pioneer in the field of autophagy research won the Nobel price: Yoshinori Ohsumi, a Japanese researcher, whose findings “led to a new paradigm in our understanding of how the cell recycles its content”.

The process of Autophagy

Fig 1: The process of autophagy

Autophagy (Autophagocytosis) describes the fundamental catabolic mechanism during which cells degrade dysfunctional and unnecessary cellular components. This process is driven by the action of lysosomes and promotes survival during starvation periods as the cellular energy level can thus be maintained.

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Supplying Discovery Tools

SQSTM1 and autophagy

09/08/2016 by Philippe Fixe, PhD No Comments

Immunofluorescence of monoclonal antibody to SQSTM1 on HeLa cell - Abnova at tebu-bio.com

Immunofluorescence of monoclonal antibody to SQSTM1 on HeLa cell. [antibody concentration 10 ug/ml] (Cat No. H00008878-M01).

Sequestosome-1 is a protein encoded by the SQSTM1 gene in humans, that is included in the autophagosome, and targets other proteins that bind to it for selective autophagy.

Autophagy was first described in the 60s, and it presents clear differences vs apoptosis. While apoptosis is a mechanism that kills the cells (apoptosis = self-killing), autophagy is more related to the orderly degradation and recycling of cellular components (autophagy = self-eating).

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Cell Biology and Signalling

How to manipulate and measure Autophagy?

20/04/2015 by Ali El Baya, PhD No Comments

The term Autophagy was introduced by Christian de Duve during the Ciba Foundation Symposium on Lysosomes – which was held in London in February 1963. In 1974 he was honoured with the Nobel price in Physiology or Medicine for his pioneering research about peroxisomes and lysosomes.

In this post, I’d like to give you an overview of autophagy and its implication in cell biology, and tools to manipulate and detect autophagy in cells.

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