Typical Standard Curves of Our New PGE2 EIA Kit (Cat. number 183K051-H1).

PGE₂ is a potent lipid mediator involved in maintaining homeostasis but also in the promotion of acute inflammation or immune suppression in chronic inflammation and cancer. Recently, researchers have shown that the activation of the NLRP3 inflammasome in human primary monocyte-derived macrophages is inhibited by PGE₂ (1) confirming the role of this lipid is an effective autocrine and paracrine inflammatory modulator maintaining homeostasis during the resolution phase of inflammation.

This paper also proove the importance of having access to simple but robust PGE2 quatitative assays.

Which Prostaglandin E2 (PGE2) Immunoassay kit to choose?

The Prostaglandin E2 (PGE2) Immunoassay kit is designed to quantitatively measure PGE2 present in serum, plasma, urine, saliva, cells, tissue, and tissue culture media samples. This
EIA kit allows for the widest variations in sample size, sensitivity and assay timing of any PGE2 kit:

• Super Fast Kinetics – Same Assay Range at 2 or 16 hours
• Incredible Sensitivity – < 2 pg/mL
• Superior Cross Reactivity to Other Eicosanoids
• Super Low Sample Volume Requirements
• Choice of Incubation Time, Sensitivity, and Sample Volume in a Single Kit

Want to test this kit allowing you to accomplish any PGE₂ measurement you may have, do not hesitate to contact us!

References

1.- Sokolowska, M. et al. (2015). The Journal of Immunology, vol. 194 no. 11 5472-5487. doi: 10.4049/​jimmunol.1401343.

Chronic Mucosal Inflammation is the hallmark of common airway diseases (ex.  Allergic Rhinitis and asthma). Lipoxin B₄(LXB₄) is an endogenous mucosal protective mediator decreasing such diseases. LXB4 mechanisms of action remain poorly understood.

Cytokine Quantibody Arrays from Raybiotech and tebu-bio laboratories.

In a recent paper in Mucosal Immunology (Karra, L. et al. (2015) 8; DOI:10.1038/mi.2014.116), Allergic Rhinitis  and asthma murine models have been described to better investigate the role of LXB4 in Mucosal Inflammation. The authors conclude that, in the upper airway, LXB₄ significantly decreases nasal mucosal leukocytes and degranulation of Mast Cells (MCs) and Eosinophils. They also show that, in the lower airway, LXB₄ significantly decreased airway inflammation, mucus metaplasia, and hyper-responsiveness.

The IL-1 family has long been known as a key player in the effector role of macrophages in innate defence against tumours and infections. It plays an important role in the field of natural / innate immunity effector mechanisms, especially as far as macrophage and macrophage-derived cytokines are concerned, in defence response. It also seems to be involved in pregnancy and pre-eclampsia (1), as well as cardiovascular diseases and type 2 diabetes (2). It is also involved in the response to Carbon Nanotubes (CNTs) exposure, where IL-1 family members activate the JNK pathway, but not ERK (3).

Fans of the series Dr. House know that in most episodes, lupus is one of the diseases considered as a tentative diagnosis by Dr. House’s team. In fact, it’s quite nice when you see how they go to the laboratory and try to check whether this possibility is true or not by looking at different biomarkers. Even TV series show the need for accurate biomarkers allowing to make an early diagnosis.