2015 was marked by the¬†increasing number of studies related to cellular¬ cytoskeletal dynamics. In this context, it should be noted that the¬†new generation of live cell-imaging SiR-Actin & SiR-Tubulin stains¬†have been widely embraced¬†by Life Scientists. Launched a few months ago, these fluorescent probes¬†are now regularly¬†cited¬†in the “Material & Methods” section of numerous prestigious publications as indicated¬†by Ali (see his the posts “Live cell imaging tool SiR-actin on JBC frontcover” and “User experience of SiR-Actin and SiR-Tubulin Live Cell Imaging“).
Nevertheless, cytoskeletal interactions are still¬†widely studied in¬†“fixed” cells. In the same manner¬†as for live cell-imaging, these experimental assays¬†require¬†high quality reagents too. With the help of our friends from Cystoskeleton Inc., we have selected 9 premium research¬†tools¬†validated for actin visualization in PFA-fixed cells. [Read more…]