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Research areas
    ADME-Tox
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    Cell Sourcing - Cell Culture Technologies
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    Gene Expression - Molecular Biology
    Stem Cells
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Tebubio's blog - Acting and reacting in life sciences and biotechnologies
  • Home
  • Research areas
    • ADME-Tox
    • Biomarkers
    • Cell Biology and Signalling
    • Cell Sourcing – Cell Culture Technologies
    • Drug Discovery
    • Gene Expression – Molecular Biology
    • Stem Cells
    • Supplying Discovery Tools
  • Contact us
  • Meet the authors
Supplying Discovery Tools

10 Mitochondrial marker antibodies

27/02/2016 by Philippe Fixe, PhD 2 Comments

There is a high demand for selective fluorescent probes for the imaging of particular organelles and biomarkers in living cells. This is particularly the case for mitochondrial research. In this post, we will focus on another type of powerful research tools (ie. primary antibodies) that have been proven to be very useful for studying mitochondria. The 10 antibodies highlighted here have been successfully used by our clients for immunoblotting of mitochondrial proteins and are all covered by tebu-bio’s Satisfaction Guaranteed.

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Cell Biology and Signalling

Tubulin:Microtubule ratio measured in cells

26/02/2016 by Ali El Baya, PhD No Comments
After a series of tubulin related blogs which were dedicated to Versatile measurement of protein binding to microtubules and Tubulin polymerisation measurement – made easy!, today I’d like to concentrate on a “in cell” approach: How can you measure changes of the ratio between free tubulin and filamentous microtubules in cells?

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Supplying Discovery Tools

Why and how to inhibit proteases?

25/02/2016 by Ali El Baya, PhD 1 Comment

There are approximately 600 proteases encoded by the human genome; countless others are produced by other animals, plants, bacteria, viruses, and yeast.

As proteases are involved in all kind of cell biological processes and play a role in a huge number of diseases, protease inhibitors are commonly used tools in essentially every Life Science laboratory.

What are the fields of applications for protease inhibitors?

Often protease inhibitors are used as simple tools to protect proteins from proteolytical degradation during protein purification or cell/tissue extract preparation. These can be standalone inhibitors or mixtures. Members of this group of nonspecific inhibitors (Leupeptin, Pepstatin A, etc.) are combined to target all protease categories: aspartic, serine, cysteine, threonine, thermolysin-like proteases, metalloproteases, and aminopeptidases.

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Biomarkers

Find metastatic biomarkers in osteosarcoma!

by Ana Arraztio No Comments

Early in 2015, researchers of The University of Queensland Diamantina Institute (Australia) have shown a very sensible approach to the discovery of new biomarkers associated to transition from non-metastatic tumours to metastatic tumours in osteosarcoma. Not to be a spoiler, but they found that the uPA/uPAR axis is crucial for this, and can be used as a prognostic biomarker. In fact, inhibition of this axis can inhibit the metastasis in this type of tumours. (Endo-Muñoz et al. DOI: 10.1371/journal.pone.0133592).

journal.pone.0133592.g006

Model of uPA/uPAR signaling in osteosarcoma and the bone microenvironment. Source: Liliana Endo-Munoz; DOI: 10.1371/journal.pone.0133592.

I don’t want to focus on the biomarker per se, but rather, on the process that this lab followed to discover this new biomarker.

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