Gene knock-out, which is gene editing leading to loss of function, just requires delivering into cells the 2 CRISPR system actors: the CAS9 endonuclease and the specific guide RNA to target the gene of interest. Thus, simple transfection of CleanCap CAS9 mRNA and gRNA followed by cell isolation with the smart aliquotor is a convenient and robust method. Howevery, what can you do if the cells are hard-to-transfect?
While Raybiotech recently released their 2000 target antibody array, a publication from “The Scientist” highlighted the benefit of these High-density multiplexed technology in biomarker discovery. As a perfect complement to traditional techniques like Mass Spectrometry, these antibody arrays provide an interesting alternative for low abundance targets, with a sensitive and high throughput approach. tebu-bio laboratories has been appointed by RayBiotech as one of the 3 companies worldwide to perform contract research on these arrays, and can take your project on board from sample processing to data analysis. This post summarizes the services available, from protein profiling, to phosphorylation and glycosylation studies, including biostatistics support. It’s worth noting that all the arrays listed are compatible with most laser scanners and can be processed in any lab.