Progesterone derived Allopregnanolone (ALLO) is an extensively studied neurosteroid that has been shown to be involved in neurodegenerative diseases, including Alzheimer’s, Parkinson’s disease, and multiple sclerosis. It is also linked to neuroinflammatory processes through a beneficial action on pro-inflammatory cytokines.

Allopregnanolone detection kits based on polyclonal antibodies were already available through Arbor Assays (K044-H and K044-C), a specialist of immunassay kits, with a special focus on inflammation and reproduction targets.

A new Allopregnanolone EIA kit has just been released, based on a monoclonal antibody (K061-H1). In addition to the supply stability offered by the monoclonal antibodies, this new kit is more sensitive than the original one, it requires smaller sample volumes and provides increased signal. It also has a more favorable cross reactivity profile:

JAK/STAT Pathway – Courtesy of RayBiotech

The JAK/STAT pathway is an important signalling process for numerous cytokines and growth factors.

Its activation is essential to many processes such as hematopoiesis, immune development, growth, adipogenesis, mammary gland development…

Galectin-3 (Mac-2) is a β-galactoside-binding lectin expressed in a variety of tissues and cell types (incl. activated macrophages, eosinophils, mast cells, dendritic cells, kidney cells, and sensory neurons).
Galectin-3 is a promiscuous protein modulating a diverse set of cellular functions such as cell adhesion, cell-matrix interactions, cell migration, macrophage activation, and apoptosis.

PD-1/NFAT-Luciferase reporter jurkat cell line used to monitor the T cell response inhibition via PD-1 interacting with PD-L1/PD-L2

PD-1 / NFAT Reporter – Jurkat Cell Line

The PD-1 reporter cell line is a T cell line expressing luciferase under the control of NFAT response elements. The level of the luciferase activity measured with the One-Step luciferase detection system (BPS Bioscience) corresponds to the T cell activation in response to the TCR activator from the target cell.

To escape, the target cell may present PD-L1 or PD-L2 to PD-1. Indeed, the interaction inhibits the T cell activation. It leads to decrease in luciferase activity.