JAK/STAT Pathway – Courtesy of RayBiotech

The JAK/STAT pathway is an important signalling process for numerous cytokines and growth factors.

Its activation is essential to many processes such as hematopoiesis, immune development, growth, adipogenesis, mammary gland development…

Galectin-3 (Mac-2) is a β-galactoside-binding lectin expressed in a variety of tissues and cell types (incl. activated macrophages, eosinophils, mast cells, dendritic cells, kidney cells, and sensory neurons).
Galectin-3 is a promiscuous protein modulating a diverse set of cellular functions such as cell adhesion, cell-matrix interactions, cell migration, macrophage activation, and apoptosis.

PD-1/NFAT-Luciferase reporter jurkat cell line used to monitor the T cell response inhibition via PD-1 interacting with PD-L1/PD-L2

PD-1 / NFAT Reporter – Jurkat Cell Line

The PD-1 reporter cell line is a T cell line expressing luciferase under the control of NFAT response elements. The level of the luciferase activity measured with the One-Step luciferase detection system (BPS Bioscience) corresponds to the T cell activation in response to the TCR activator from the target cell.

To escape, the target cell may present PD-L1 or PD-L2 to PD-1. Indeed, the interaction inhibits the T cell activation. It leads to decrease in luciferase activity.

Human and Animal Dermal S9 Fractions now available as standard products

Subcellular fractions are widely used in drug discovery and preclinical drug development to evaluate the in vitro metabolism of new therapeutics. Skin fractions are already available from tebu-bio as catalogue items for CD1 Mouse and IGS Sprague Dawley Rat.

In collaboration with Sekisui XenoTech, tebu-bio is now adding human and minipig dermal subcellular fractions to the list of species available as standard test systems.