In the last few decades, in the field of Drug Discovery, Immunotherapy has gained more and more importance for the treatment of various diseases and in particular for certain types of cancer. Based on inducing, enhancing or suppressing an immune response, this therapeutic manipulation has led to promising clinical results.

Major regulators of Immune activation, Immune checkpoints play a key role in maintaining immune homoeostasis and preventing autoimmunity. However in some cancers, these checkpoints can be used to avoid any activation of the immune response against cancer cells. Immunotherapies based on targeting immune checkpoint pathways in Drug Discovery, have already led to the development of promising molecules (Nivolumab, Pembrolizumab…) tested in clinical trials and approved, which are giving new hope for cancer treatments.

Undoubtedly, producing and purifying a protein is an art form.

Indeed, each recombinant protein (rec. protein) to be produced in vitro has specific characteristics and singularities, which makes its production and purification absolutely challenging. In this post, I’d like to share some tips and review basics for optimizing experimental design when producing functional recombinant proteins and working on protein purification.

Since 2000, tebu-bio provides protein services from production to purification, we can help you further determine the most appropriate strategy by considering all parameters and following experimental requirements.

By mimicking in-vivo environments, 3D cell culture models appear nowadays as the best in-vitro study model to work in an in-vivo like study model and to obtain more physiologically relevant data and proof of concept as close as possible to “a clinical context” (aka the “near-human” approach). To accompany researchers along this way, there are several innovative cell culture devices (available in Europe through tebu-bio), and more specifically a modular microfluidic platform for 3D cell culture with the capability to monitor complex biological systems dynamics in response to tuned microenvironment: the 3D Cell culture chip (Aim Biotech)

If your research is related to obesity and adipose tissue, you may be interested by the selection I have performed below of immunoassays (including ELISA tests and antibody arrays), as well as primary cell models related to this topic.

Obesity and the diseases arising from it (diabetes, cardiovascular disease and cancer) constitute a major health challenge to the industrialized world. In the past decade, it has become apparent that adipose tissue is a major endocrine organ which secretes numerous soluble factors and regulates many processes related to glucose homeostasis, lipid and protein metabolism, inflammatory and immune responses…