ORF expression in Mammalian cells is very useful for many applications: protein production with HEK293, over-expression of tagged protein for immuno-tracking or immuno-precipitation, development of a cell line for drug discovery such as a cancer model expressing PD-1 or PD-L1…

Unfortunately, plasmid delivery into primary cells and cell lines is often challenging, providing low or even no transfection efficiency. The solution is a robust lentiviral system.

So, how do you identify the right lentiviral system? With the titer! The higher is the better. 10⁷TU/ml is ok… and 10⁸TU/ml is very good.

Due to their ability to simulate the characteristics of most soft tissues and natural scaffolds, hydrogels are now an attractive material for developing synthetic Extracellular Matrix (ECM) analogs.

Recently, the VitroGel™ hydrogels have been designed for researchers willing to fully master the characteristics of the synthetic ECM to be used with their 3D / 2D cell cultures. In this post, we will review the main benefits of the recently released VitroGel™ solutions.

Nephropathy is a hot research topic, especially in relation to its link with Diabetes (already 6 500 publications so far referenced in pubmed to date for 2019, a third of them in relation to type I or type II diabetes). As nephropathy can lead to life endangering end-stage renal diseases (ESRDs) with a severe impact on life expectancy, a lot of research is being undertaken to find new drugs to beat this potentially severe condition. Several biomarkers of this pathology have already been identified such as CD27 antigen, kidney injury molecule 1 (KIM-1) and α1-microglobulin in relation to type I diabetes (1), in addition to the well known Clusterin, Cystatin-C, Microalbumin and Beta-2 Microglobulin.

Thanks to its capability to recruit the natural protein degradation system of a cell, PROTACs (PROteolysis TArgeting Chimera) appears as a very promising technology in cancer therapy and disease treatment. As a distributor of innovative reagents for life science researchers, tebu-bio provides you with all the necessary tools to develop or test your own PROTACs system.