Since the discovery of reprogramming factors in 2006 and the boom of CRISPR gene editing strategies, induced pluripotent stem cells (iPSC) have emerged as new cellular models. The development of 3D cell culture technologies has also contributed to the generation of induced Pluripotent Stem Cell (iPSC) derived cells, with unique applications from patient-specific drug responses testing, to regenerative medicine. I would like to introduce in this post a selection of reagents in this domain, a combination of both routine and innovative quality reagents, that I consider as bringing something extra to your stem cell research projects.

Cell-Based ELISA tests now enable precise measurement of the phosphorylation status or expression profiles of target proteins (biomarkers) in whole cells. In this post, a review of high performing cell-based assays is made.

In one of my previous posts, I wrote about high density glass slide antibody arrays services.

Here, I’d like to focus on another antibody microarray product line, the C-series from Raybiotech, based on a membrane antibody array technology. Similar to western blotting – therefore very easy to process – they are suitable to most research laboratories for targeted protein profiling.

Signal-Seeker assays (Cytoskeleton Inc.) provide researchers with the most comprehensive reagents enabling highly sensitive and specific detection of the key Post Translational Modifications (PTMs) of your Protein of Interest.

In this post, I invite you to look at experimental results obtained with these unique assays for the characterization of Tyrosine Phosphorylation, Ubiquitination, SUMOylation and Acetylation of endogenous proteins.