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tebu-bio's blog - Acting and reacting in life sciences and biotechnologies
  • Home
  • Research areas
    • ADME-Tox
    • Biomarkers
    • Cell Biology and Signalling
    • Cell Sourcing – Cell Culture Technologies
    • Drug Discovery
    • Gene Expression – Molecular Biology
    • Stem Cells
    • Supplying Discovery Tools
  • Contact us
  • Meet the authors
Supplying Discovery Tools

How do you maximize immunoprecipitation?

30/04/2018 by Dimitri Szymczak, PhD No Comments
Co-immunoprecipitation

Protein purification from cells or supernatants, endogenous proteins complex analysis, and targeted protein enrichment for even more sensitive mass spectrometry-based biomarker discovery requires a particular immunoassay called immunoprecipitation (IP). Basically, the most robust method to pull-down a specific protein with its partners from cell lysates should be the combination of an antibody conjugated to biotin and magnetic beads with streptavidin. Unfortunately, if your antibody is not yet conjugated to biotin, conjugation is a blind process. That is why, using protein A/G coated sepharose beads is the most popular method.

But what if an antibody biotin conjugation kit could provide simple UV-scan based quantification of the conjugation yield?

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Cell Sourcing - Cell Culture Technologies

AACR2018 – tebu-bio presents “Time-lapse analysis of drug response and invasive capacity…”

26/04/2018 by Sara Pierre No Comments
"Time-lapse analysis of drug response and invasive capacity using patient-derived and CRC microfluidic 3D cancer models under oxygen controlled conditions"

This year again, we were pleased to attend the Annual meeting of the AACR (American Association for Cancer Research), which took place in Chicago, USA earlier this month.

Dora Sabino PhD of tebu-bio at the AACR2018 Poster Session
Dora Sabino PhD of tebu-bio at the AACR2018 Poster Session
Dora Sabino PhD of tebu-bio at the AACR2018 Poster Session
Dora Sabino PhD of tebu-bio at the AACR2018 Poster Session
Dora Sabino PhD of tebu-bio at the AACR2018 Poster Session
Dora Sabino PhD of tebu-bio at the AACR2018 Poster Session

Dora Sabino Ph.D. (3D Cell Culture Project Manager, tebu-bio) presented a poster covering a physiologically relevant study on 3D colon cancer models in microfluidic chips:

“Time-lapse analysis of drug response and invasive capacity using patient-derived and CRC microfluidic 3D cancer models under oxygen controlled conditions”

Download your copy of the poster here and learn more about tebu-bio’s laboratory outsourcing solutions.

Cell Biology and Signalling

Cell signalling studies: Tips for selecting the best Small G Protein activation assay

25/04/2018 by Frédéric Samazan No Comments

Small GTP-binding proteins such as Rho and Ras GTPase family members are involved in regulating cell signalling pathways and impact a wide range of cellular processes, functions, and morphology. In this post, you’ll discover two types of Small G-protein Activation Assays to easily measure the GTP-bound form of your protein of interest, as well as some tips for choosing between both solutions and finding the assays that fit best with your cell signalling experiments.

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ADME-Tox

Assess catabolic stability of biologics & ADCs with Lysosomes – Characterised test systems

24/04/2018 by Isabelle Nobiron, PhD 4 Comments

Following up on my series of posts based on Dr Chris Bohl’s work at Sekisui Xenotech, in this post I invite you to take a look at the work he has published, together with Dr Christopher Seib, Maciej Czerwinski, Zell Woodworth and David Buckley, Ph.D., illustrating the characterization of isolated human liver lysosomes, and validating them as test systems for in vitro assessment of catabolic stability of biologics drugs entering the cell by the endosomal–lysosomal pathway.

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