In a previous post, I introduced a complete panel of ready-to-use specialized media for smooth muscle primary cells culture. Produced by Cell Applications, a GMP certified manufacturer of primary cells distributed by tebu-bio in Europe, these media were originally designed for Cell Applications own primary cells culture. However, many researchers isolating their own cells are using them to benefit from the expertise of a primary cell manufacturer and to use thoroughly QC tested media. Here, I invite you to look at a new selection for popular primary cell types, from skeletal muscle cells to synoviocytes, endothelial cells to pre-adipocytes. Have a read and find the one you need!

For all species, classes or size (FL vs. fragments)

Whatever the type of antibody production (hybridoma or recombinant protein production in bacteria or in mammalian cells), you need to isolate your antibody of interest from other proteins present in the sample (sera, ascites fluid, medium, cell lysate…).

In order to isolate the antibody of interest, you need to select the right strategy depending on the nature of the antibody (species, class, tagged or not), the purity required and the intrinsic antibody physico-chemistry.

Since 2000, tebu-bio provides antibody services from production to purification, we can help you further determine the most appropriate strategy by considering all parameters, including intrinsic antibody characteristics and following experimental requirements.

This article is your first guide to prepare the best angle of attack to put into action!

Chromatin profiling assays enable the mapping of protein-DNA interactions that influence chromatin modeling and gene expression.
Over the years, Chromatin ImmunoPrecipitation (ChIP) assays became the gold-standard for studying transcription factor–DNA binding interactions, nucleosome dynamics, and epigenetics (ex. histone post-translational modifications (PTMs)). This post aims at introducing the latest evolution of ChIP assays: CUTANA™ CUT&RUN and ChIC assays.

Over the last years, ChIP experts express the need for more sensitivity, faster protocols, and more reproducible results (and ideally starting from less starting cellular material). These expectations led to the emergence of new experimental antibody-based experimental approaches like Chromatin ImmunoCleavage (ChIC) assays, and Cleavage Under Targets and Release Using Nuclease (CUT&RUN) methods.

Multiplex protein assays allow multiple targets quantification in a single sample, a bit like performing up to 40 different ELISAs in one go. Outsourcing Multiplex Protein Quantification projects to experts may work out more cost effective and time saving. It saves you the hassle of setting up a new technology in your lab, and you can rely on people with years of expertise in the technology to generate quality data on the very first run. Should you wish to set it up in your own lab, some technologies are easily adaptable if you have access to the equipment.