My colleague Ana introduced in a previous post (3 years ago already!) a selection of the most popular anti-tag antibodies among the research community. I must say that they remain extremely popular, showing their robustness over time. In this post, I would like to focus on anti-RFP antibodies and anti-GFP antibodies, looking at a selection of high quality, fully validated, reliable tools.

Cell viability after cryopreservation is a sensitive subject as it may be dependent on the ability of mammalian cells to face the stress generated by the freeze / thaw cycles. Improving cell viability whilst maintaining optimal cellular functions and pathways has thus become a challenge. In this post, we invite you to take a look at 3 optimized reagents to improve cellular viability after cryopreservation with serum-free and protein-free CryoStor cryopreservation freeze media solutions.

Sekisui XenoTech have announced the addition of Rodent Hepatocytes to their patented CryostaX® product line.

The CryostaX® in vitro drug discovery and preclinical drug development test system is continuously expanding with these new pooled Sprague-Dawley rat hepatocytes  released in July, and pooled CD-1 mouse hepatocytes soon to be launched.

CryostaX® hepatocytes are liver cells that are prepared using a patented process that produces unique cryopreserved cell pellets. This single-freeze preparation process provides greater convenience to researchers, versatile pooling options, and minimizes freeze-thaw injury to the cells, which promotes high viability, cell yield and enzymatic activity for longer-term, consistent test results. What else makes Cryostax a popular choice for researchers?

With their polycationic properties, polyamine organic compounds are involved in various biological functions (DNA-RNA interaction, gene expression…), and can also be considered as cancer marker.

In this post, I’m pleased to introduce the first intra-cellular polyamine imaging reagent which works without any pre-treatment or cell lysis. This innovative reagent can be used with fixed cells, thus making it suitable for detection or semi-quantitative analysis of intra-cellular polyamines.