When scientists at ArborAssays invented the Direct Cyclic Nucleotide EIA immunoassay more than 20 years ago, they knew the kits would be popular. The direct nature of the format for the assays allows cAMP & cGMP in cells to be measured in one step. They went on to refine the assay, developing both colorimetric and chemiluminescent versions for cAMP and cGMP. But what makes them so popular?

As is well known, the actin cytoskeleton is a highly dynamic structure, involved in a large number of cellular processes, including muscle contraction, lamellopodial extrusion, cell locomotion, cytokinesis, intracellular transport and many more. Under the tight regulation of more than 50 actin binding proteins (ABPs) and in response to a wide variety of internal and external stimuli, the morphology of the actin cytoskeleton changes rapidly.

For more than 20 years now, Cytoskeleton Inc. has provided a large selection of robust and user friendly solutions (pure and active actin proteins/Biochemical assays and actin staining probes..) which have helped many researchers to solve fundamental questions in cell signalling, cytoskeleton and actin research fields.

Neurotrophic factors are known to protect neuronal cell death in several neurodegenerative diseases, both in in vitro and in vivo. They play a key role in development, differentiation, synaptogenesis, and survival of neurons in the brain as well as in the process of their adaptation to external influences.  As such, they are essential growth factors in neuroprogenitor cell culture in vitro (Pluripotent stem cells). A sustained release of such factors in culture can be a challenge. Encapuslating them in polymer beads is an answer to this issue, as shown in this post introducing new StemBeads for BDNF and GDNF.

When compared to 2D conventional cell culture systems, experimental data obtained with 3D cell culture models have been shown in many instances to be more biologically relevant, closer to clinical conditions (eg. predictive assays/preclinical therapeutic testing).

In this context, tebu-bio laboratories have developed a spheroid platform with a selection of downstream analysis applications such as live cell imaging, microfluidic and physioxia culture conditions or downstream multiplex biomarker quantification, among others.

If you need more physiologically relevant models for your study, but you don’t have access to the technologies or haven’t the time to spend on setting up spheroid cultures in your own laboratories, read on to see what tebu-bio laboratories can generate for you in terms of data.