In 2010, a method for integration-free reprogramming by transfecting modified mRNA reprogramming factors was published (1, 2). The use of mRNA to induce reprogramming of somatic cells overcomes the inherent problems incurred by introducing viral vectors and/or integrating DNA to target cells. mRNA reprogramming factors are titratable and controllable with regards to the ratios, concentrations, and timing of factor expression. The mRNA reprogramming system also poses none of the biosafety risks surrounding viral and DNA-based systems.
The Institut Pasteur (Paris, France) announces an international call for candidates wishing to establish independent research groups as a part of the Revive Laboratory of Excellence (LabEx) programme on “Stem Cells and Regenerative Biology and Medicine”.