For all species, classes or size (FL vs. fragments)

Whatever the type of antibody production (hybridoma or recombinant protein production in bacteria or in mammalian cells), you need to isolate your antibody of interest from other proteins present in the sample (sera, ascites fluid, medium, cell lysate…).

In order to isolate the antibody of interest, you need to select the right strategy depending on the nature of the antibody (species, class, tagged or not), the purity required and the intrinsic antibody physico-chemistry.

Since 2000, tebu-bio provides antibody services from production to purification, we can help you further determine the most appropriate strategy by considering all parameters, including intrinsic antibody characteristics and following experimental requirements.

This article is your first guide to prepare the best angle of attack to put into action!

Chromatin profiling assays enable the mapping of protein-DNA interactions that influence chromatin modeling and gene expression.
Over the years, Chromatin ImmunoPrecipitation (ChIP) assays became the gold-standard for studying transcription factor–DNA binding interactions, nucleosome dynamics, and epigenetics (ex. histone post-translational modifications (PTMs)). This post aims at introducing the latest evolution of ChIP assays: CUTANA™ CUT&RUN and ChIC assays.

Over the last years, ChIP experts express the need for more sensitivity, faster protocols, and more reproducible results (and ideally starting from less starting cellular material). These expectations led to the emergence of new experimental antibody-based experimental approaches like Chromatin ImmunoCleavage (ChIC) assays, and Cleavage Under Targets and Release Using Nuclease (CUT&RUN) methods.

Multiplex protein assays allow multiple targets quantification in a single sample, a bit like performing up to 40 different ELISAs in one go. Outsourcing Multiplex Protein Quantification projects to experts may work out more cost effective and time saving. It saves you the hassle of setting up a new technology in your lab, and you can rely on people with years of expertise in the technology to generate quality data on the very first run. Should you wish to set it up in your own lab, some technologies are easily adaptable if you have access to the equipment.

ORF expression in Mammalian cells is very useful for many applications: protein production with HEK293, over-expression of tagged protein for immuno-tracking or immuno-precipitation, development of a cell line for drug discovery such as a cancer model expressing PD-1 or PD-L1…

Unfortunately, plasmid delivery into primary cells and cell lines is often challenging, providing low or even no transfection efficiency. The solution is a robust lentiviral system.

So, how do you identify the right lentiviral system? With the titer! The higher is the better. 10⁷TU/ml is ok… and 10⁸TU/ml is very good.