Neurotrophic factors are known to protect neuronal cell death in several neurodegenerative diseases, both in in vitro and in vivo. They play a key role in development, differentiation, synaptogenesis, and survival of neurons in the brain as well as in the process of their adaptation to external influences.  As such, they are essential growth factors in neuroprogenitor cell culture in vitro (Pluripotent stem cells). A sustained release of such factors in culture can be a challenge. Encapuslating them in polymer beads is an answer to this issue, as shown in this post introducing new StemBeads for BDNF and GDNF.

When compared to 2D conventional cell culture systems, experimental data obtained with 3D cell culture models have been shown in many instances to be more biologically relevant, closer to clinical conditions (eg. predictive assays/preclinical therapeutic testing).

In this context, tebu-bio laboratories have developed a spheroid platform with a selection of downstream analysis applications such as live cell imaging, microfluidic and physioxia culture conditions or downstream multiplex biomarker quantification, among others.

If you need more physiologically relevant models for your study, but you don’t have access to the technologies or haven’t the time to spend on setting up spheroid cultures in your own laboratories, read on to see what tebu-bio laboratories can generate for you in terms of data.

The main challenge in RNA-sequencing workflow is certainly library preparation. When it’s question of single-cell isolation and so 10-100 picogram of total RNA, the challenge may become mission impossible… Today, we’re pleased to present a smart and simple device, to solve both single cell isolation and to help with library preparation.

Single-cell isolation allows genomic and transcriptomic analysis of one individual cell. It is also required to build a monoclonal cell line from rare cell isolation, that could be for example CRISPR-CAS9 gene edited cells. There are 3 popular methods: serial dilution, micro-manipulation and flow cytometry. None of these are easy or simple, and they often require expertise and experience. Fortunately, a new solution for everybody has come! It’s designed to isolate a single cell in a few seconds, and it’s (quite appropriately) called the Smart Aliquotor.