RayBiotech , the antibody array specialist, have just released a new, very broad antibody microarray, the L2000 series, for the profiling of 2000 protein targets including metabolic enzymes, structural proteins, and a variety of cytokines, growth factors, proteases, and signaling proteins. Based on the same technology as the “smaller” version L1000, the L2000 array is the quickest and simplest method to profile extensive protein expression with small sample volume. In this post, we’ll look at the array’s features and technology, as well as the possibility to outsource your profiling project to experts in Europe, particularly for one shot experiments, to save you the time to set up the technology in your lab whilst securing the quality of your data.

If your research interest is focused on the identification of cell signalling pathways and protein phosphorylation profiles, the comprehensive Phospho Explorer Array may be of great help to you, measuring changes in phosphorylation status in more than 1000 targets at once. I invite you to take a look at the technology and its benefits, after which I’ve included some publications in 2018 to give you an idea of how other researchers have used this array and how it helped them generate meaningful data in their phosphorylation studies.

Antibody generation raises a number of questions… what is the difference between their classes, forms and types? And how do recombinant monoclonal antibodies (rAbs) overcome the drawbacks of classical monoclonal antibodies (mAbs)?

Monoclonal antibodies are ubiquitous in biomedical research and medicine. They are used to fight, diagnose and research disease and to develop and test new drugs. The antibodies are divided in 5 classes or isotypes, several subtypes and forms and can be generated in vivo or in vitro.

In this short article, my aim is to define what an antibody is, to highlight the differences between a hybridoma monoclonal antibody and a recombinant monoclonal antibody and to point out how rAbs bring solutions to the classical drawbacks of mAbs.

As they say, a good picture is worth a thousand words. That’s to say that I’ll have to keep the introduction short in this post (!) to let you know about this interesting map from Boster Bio summarizing the allergic response pathway. I’ll take this opportunity to mention a few related immunoassays that may help you deciphering this pathway in your own study!