RNA-based CRISPR-CAS9 gene editing is a vector-free approach that is required for therapeutic perspectives. However, from a practical point of view, what appears as a major challenge to engineer primary cells, is actually also an efficient and smart solution for numerous other knock-out projects on cell lines. We see here why and how.

Cell-based assays, screening for pathway activation or inhibition are classically done with promoter reporter clones expressing Firefly luciferase. Brighter, more stable, more sensitive, more convenient and cheaper, Gaussian luciferase has become popular over the past years. Let’s discover here the next generation of reporter clones, called Gluc-ON.

RayBiotech , the antibody array specialist, have just released a new, very broad antibody microarray, the L2000 series, for the profiling of 2000 protein targets including metabolic enzymes, structural proteins, and a variety of cytokines, growth factors, proteases, and signaling proteins. Based on the same technology as the “smaller” version L1000, the L2000 array is the quickest and simplest method to profile extensive protein expression with small sample volume. In this post, we’ll look at the array’s features and technology, as well as the possibility to outsource your profiling project to experts in Europe, particularly for one shot experiments, to save you the time to set up the technology in your lab whilst securing the quality of your data.

If your research interest is focused on the identification of cell signalling pathways and protein phosphorylation profiles, the comprehensive Phospho Explorer Array may be of great help to you, measuring changes in phosphorylation status in more than 1000 targets at once. I invite you to take a look at the technology and its benefits, after which I’ve included some publications in 2018 to give you an idea of how other researchers have used this array and how it helped them generate meaningful data in their phosphorylation studies.