I just want to briefly outline in this post the power of antibody microarray when it comes to time and cost saving as well as versatility. Whether you want to do multiple profiling or quantitative studies, if you have access to only limited sample volume, if you work with “rare samples”, if you have access to laser scanner or just to a CCD camera… there is one format suited to your multiplex protein study. The whole experiment is extremely easy to perform but nothing stops you from outsourcing it to experts for the price of an extra ELISA :=). I picked as example for this post the popular inflammation arrays to illustrate the technology and give you some price examples to convince you that it’s not more expensive than an ELISA!

Despite its importance in the regulation of many cellular processes, membrane tension is still complicated to measure. And despite valuable insights, the techniques currently available remain complex and present technical limitations. To overcome these drawbacks, Spirochrome have developed the first probe for fluorescence microscopy in the field of mechanobiology, that specifically targets the plasma membrane of cells and reports membrane tension through changes in its fluorescence lifetime : the Flipper-TR^® probe.

As ADME in vitro models are not just about human, mouse or rat hepatocytes and liver cell fractions, it’s important to have access to readily available sourcing for more seldom used models or tissues. Sekisui Xenotech can easily meet these demands with their Custom Products Division who has extensive experience preparing hepatocytes, subcellular fractions and other cells from a wide variety of species and tissue. The project can be fully customized for your particular needs, with the species, organ and characterization of your choice. Alternatively, you can benefit from the surplus list from previous extractions at competitive pricing and short time frame. I tell you a bit more about the 2 options in this post, and give you direct access to the current surplus inventory list. You may find what you are looking for in there!

Optimal knock-down can be done by a shRNA sequence that depends on the gene expression level and the cell type. Usually, 4 shRNA should be tested to find the one inducing a minimum of 70% increase of the targeted mRNA pool. Genecopoeia has developped shRNA expression vectors with improved performances.