Cell signalling: Where to find multiplex arrays for glycobiology studies

Glycan 300 Array RayBio fluorescent scan

How do you detect 300 specific glycan binding proteins in one experiment? Well, in this post we’ll answer this question by introducing the new Glycan Array, developed by RayBiotech for your cell signalling studies.

Glycocalyx is an extracellular polymeric coating surrounding many prokaryotic and eukaryotic cells. It consists of glycoproteins, glycolipids, proteoglycans, and glycosaminoglycans.

Glycan Array Detection principle - RayBio tebu-bio

RayBio® Glycobiology cell signalling Arrays are a versatile and innovative series of products designed to detect glycosylation.

These constituents play an important role in the processes of cell signalling, virus transfection, and immunity. However, the range of detection tools available for glycobiology research have been limited…  until now!

Cell Signalling Glycan Array: Detects 300 specific glycan binding proteins

With the Glycan Array 300 (cat. nr GA-Glycan-300-1), it is now possible to easily detect glycan binding proteins in one single experiment using the microarray technology.

RayBiotech have developed the largest commercially available glycan array for screening protein-carbohydrate interactions in cell signalling events to help you:

  • Identify the glycans’ binding partners in biological samples
  • Identify whether target proteins are carbohydrate binding proteins
  • Probe binding of viruses and whole cells to glycans
  • Profile the substrate specificity of enzymes (glycosyltransferases, glycosidases, etc.)
  • Profile the inflammatory immune response

The 300, HPLC purified, synthetic glycans featured in the new generation Glycan-300 array consists of four groups of targets including:

  1. The 100 glycans from the Raybio Glycan-100 array
  2. An additional 80 N-glycans
  3. 50 glycolipid glycans
  4. 50 human milk oligosaccharide glycans
Human Milk oligosaccharides Glycan 300 Raybiotech tebu-bio

Cell signalling studies: Human Milk Oligosaccharides (linkers & Oligosaccharide structures) in the Glycan 300 Array (RayBiotech – tebu-bio).

The included glycans have been frequently identified as structures showing important binding functions in the literature. For example:

  • A variety of sialosides that have been shown to bind to the influenza virus with a serotype-specific pattern
  • Galectins, which are involved in apoptosis, cell adhesion and T-cell activation suppression, function by binding beta-galactosides
  • Glycosphingolipids knwon to interact with growth factor receptors to modify signal transduction & cell signalling or with bacterial toxin subunits to induce cell apoptosis
  • Oligosaccharides from human milk that bind to microbes to promote growth of normal flora and inhibit pathogens in the gastrointestinal tract

This Glycan 300 array provides a powerful tool for researchers in glycobiology and other fields like cell signalling.

Other RayBio Glycobiology Arrays for your cell signalling studies

Glycan 100 Array

In addition to the Glycan Array 300, a smaller version for the detection of 100 different glycans. The 100, HPLC purified, synthetic glycans featured in the Glycan Array 100 are the most frequently identified structures showing important binding function in the literature.

Lectin Array 70 & 95

Lectins are glycan-binding proteins which have been purified from trees, beans and some fruits. They are highly specific for a given glycan based on their sequence and the different sugar unit structures the glycan contains.

The RayBiotech Lectin Array 70 & Lectin Array 95 provide a powerful tool for glycosylation determination, drug discovery and biomarker development; all with limited samples volumes required.

When (and why) should you decide to outsource your cell signalling assay?

tebu-bio: European RaybioTech's Certified Laboratory service providerApart from the obvious considerations (lack of human resources to perform your project, need to hurry up your experiments to match a deadline, lack of equipment..), there may be other reasons to rely on tebu-bio’s lab expertise to perform your Glycan profiling & biomarker quantification for you. If you are on a one shot experiment where you want to optimize your results and benefit from our expertise, outsourcing may be a better choice than doing it yourself. Although the technology is quite simple, tebu-bio’s lab is very experienced and is likely to generate better quality data than you would in a single trial.

Feel free to contact me for any further discussions about your glycan protein profiling studies.
Written by Philippe Fixe, PhD
Philippe Fixe is a former Marketing Manager at tebu-bio, passionate about innovation and R&D in Life sciences, Biotechnology, Medical research, Drug discovery, and also a keen photographer.