New HYDROP fluorescent dye for intracellular ROS measurement

visualization-of-hydrogen-peroxide-production-by-raw-264-7-cells-using-hydrop

Reactive oxygen species (ROS) play key roles in various intracellular processes and have been shown to be involved in many diseases (eg. carcinogenesis, inflammation…). Each of the ROS species is likely to have a specific role in living cells. Therefore, there is an emerging need for selectively detecting each species of ROS through conventional biochemical assays, but also in live cell imaging (see a previous post “Reactive Oxygen Species (ROS) and related assay kits“).

reaction-of-hydrop-to-various-ros-source-goryio-chemical-tebu-bio

Reaction of HYDROP to various ROS.

Recently, Goryo Chemical has launched a new fluorescent dye (HYDROP) to complement their existing range of ROSFluor™ Series, which aims at measuring ROS species in live cell imaging.

HYDROP specifically detects H2O2

HYDROP is a cell-permeable, fluorescent (exc. max: 492 nm, em. max: 518 nm) probe which is non-fluorescent under physiological conditions.

Once in the cell, HYDROP fluoresces upon reaction with hydrogen peroxide (H2O2). It shows high selectivity towards H2O2 compared to other ROS species such as the hydroxyradical (・OH), superoxide (O2-・), hypochlorous acid (OCl-), singlet oxygen (1O2), nitric oxide (・NO), and peroxynitrite (ONOO-).

HYDROP in action

In the example below, green fluorescent signal is overlayed to DIC image. Hydrogen peroxide production was induced by the addition of 0.001 µg/ml phorbol myristate acetate (PMA). Cells were stained with 1 µM HYDROP for 20 min. Bar, 25 µm.

visualization-of-hydrogen-peroxide-production-by-raw-264-7-cells-using-hydrop.  Source: Goryo | tebu-bio.

Visualization of hydrogen peroxide production by RAW 264.7 cells (1×105 cell/ml) using HYDROP.  Source: Goryo | tebu-bio.

 

Other fluorescent dyes for studying ROS in fluorescent assays

HYDROP complements an existing offer of fluorescent dyes developed by Goryo (ROSFluor™ Series):

  • HYDROP for the detection of hydrogen peroxide (H2O2)
  • Hydroxyphenyl Fluorescein (HPF) & Aminophenyl Fluorescein (APF) for the detection of hydroxyl radical (・OH), peroxynitrite (ONOO-) and hypochlorite (OCl-)
  • NiSPY-3 for the detection of peroxynitrite (ONOO-)
  • HySOx for the detection of hypochlorous acid (HOCl)

Also of interest is the silicon-based far-red fluorescent probe (Si-DMA – Dojindo), which has been especially designed to monitor singlet oxygen in real-time and in living cells (see “How to detect Singlet Oxygen by live cell imaging?”)

LCI cell cartoon

Live Cell Imaging tools available at tebu-bio

 

If you are interested in testing one of the live cell imaging stains presented, leave your request in the form below. For certain references, samples are available upon request.

You might also like to get a complete overview of tebu-bio’s unique Live cell imaging offer via an interactive Live cell imaging tool selection guide. All you need to do is just click on the biological parameter you want to visualize – and you’ll find the corresponding molecular probes available.

 

 

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Written by Philippe Fixe, PhD
Philippe Fixe is a former Marketing Manager at tebu-bio, passionate about innovation and R&D in Life sciences, Biotechnology, Medical research, Drug discovery, and also a keen photographer.