New – Continued use immunotherapy cell line expressing recombinant PD-1

T cell activation

PD-1/NFAT-Luciferase reporter jurkat cell line used to monitor the T cell response inhibition via PD-1 interacting with PD-L1/PD-L2

PD-1 / NFAT Reporter – Jurkat Cell Line

The PD-1 reporter cell line is a T cell line expressing luciferase under the control of NFAT response elements. The level of the luciferase activity measured with the One-Step luciferase detection system (BPS Bioscience) corresponds to the T cell activation in response to the TCR activator from the target cell.

To escape, the target cell may present PD-L1 or PD-L2 to PD-1. Indeed, the interaction inhibits the T cell activation. It leads to decrease in luciferase activity.

Neutralizing anti-PD-L1

Fold induction in presence of neutralizing anti-PD-L1

The inhibition of PD-1 interaction to PD-L1/PD-L2 blocks the escape of the target cell and promotes the T cell activation. It can be performed by a specific neutralizing antibodies such as the Anti-PD-1 Neutralizing Antibody and the Anti-PD-L1 (CD274) Neutralizing Antibody. The inhibition of the PD-1/PD-L1 interaction leads to the rescue of the luciferase activity coded by the NFAT-Luc reporter (Similarly shown by fold induction).

The PD-1 reporter line is the key of a cell-based assay to investigate the escape of target cell, namely cancer cells, to be killed by the T cell.

How can this help you?

This kit is definitely of interest to you to…

  • Discover the cellular pathways involved in PD-1 signaling
  • Study PD-1 modulators in a cellular context
  • Study PD-L1 or PD-L2 interaction with PD-1

The ready-to-use PD-1 reporter cell line can be propagated and stored for the long term and used many times.

For use over a limited time, one option is that they can be rented for 3 months (get in touch with me through the comments form below if this is something you’re interested in for your lab).

Similarly, you can also get a model of target cell, PD-L1 – CHO Recombinant Cell Line.

Designing the target cell

The new TCR Activator / PD-L1 Mammalian Expression Kit allows you to transfect another cell line to express the TCR activator and the PD-L1 protein. These cells can be used together with the PD-1 reporter cell line for a cellular assay of PD-1:PD-L1 binding and activation. There is also a solution for PD-L2, the TCR Activator / PD-L2 Mammalian Expression Kit.

ONE-Step™ Luciferase Assay System

The ONE-Step™ Luciferase Assay Reagent, (BPS Biosciences) generates an extended bright light signal which allows for high sensitivity, even during extended incubation periods.
This assay shows increased tolerance to different sample and reaction components and exhibits low influence from luciferase inhibitors, resulting in fewer false positive readings. Available in 10 ml and 5 x 20ml sizes, reducing prep time of screening experiments.

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Written by Dimitri Szymczak, PhD
Dimitri Szymczak is a Technical Support Specialist and Product Manager at tebu-bio, and a fan of capoeira in his spare time.