Mimic human pulmonary function in vitro
If you’re studying human pulmonary function and pathophysiology, you need access to validated, highly characterised, human airway cellular models. A large collection of these cells is available for applications in asthma, inhalation toxicology and pulmonary inflammatory response. Let’s take a look at a selection of different pulmonary cell types I would advise, to boost your in vitro research in this area.
Epithelial Cells – Bronchial & Epithelial
HBEpC & HTEpC provide an excellent model system to study all aspects of epithelial function and disease, particularly those related to airway viral infections, as well as tissue repair mechanisms, signaling changes and potential treatments relevant to lung injuries, mechanical and oxidative stress, inflammation, pulmonary diseases and smoking. HBEpC & HTEpC are obtained from healthy donors, as well as from asthma patients.
Microvascular Endothelial Cells
Human Lung Microvascular Endothelial Cells (HLMVEC) provide a useful tool for studying various aspects of pathology and biology of the pulmonary microvasculature in vitro. HLMVEC (Cell Applications, Inc.) have been used to elucidate the therapeutic effects of Angiotensin I-converting enzyme (ACE) inhibitors, and the results revealed that they provide an additional benefit to patients by activating bradykinin B1 receptor leading to prolonged nitric oxide (NO) production in endothelial cells and inhibition of PKCϵ (Ignjatovic, 2004; Stanisavljevic, 2006).
Picture: HLMVEC in culture (A). HLMVEC immunolabeled for CD31/PECAM (green); nuclei are visualized with PI (red) (B). HLMVEC transfected with GFP plasmid DNA using the Cytofect™ Endothelial Cell Transfection Kit (C,D). Courtesy of Cell Applications, Inc.