Melanoma cell lines – how to bridge gaps between patient & lab samples
Tumour modelling facilitates the study of tumour growth in the tumour microenvironment (TME). Therapies that affect the tumour microenvironment are critical for advancing the fight against cancer as emerging therapies target the network of signaling pathways essential for tumour growth, Epithelial Mesenchymal Transition and metastasis (eg. Wnt/ß-Catenin pathway). Patient derived tumour models, including melanoma cell lines, are invaluable tools to study normal and malignant cells, tumour formation and drug resistance.
Rockland Immunochemicals Inc. has partnered with the Wistar Research Institute, to produce and validate a diverse panel of low passage melanoma cell lines from freshly excised metastases. More than 100 melanoma cell lines are grouped for BRAF, N-RAS, KIT, PTEN and CDK4 mutations. These pre-clinical tumour cell lines models can be used to identify the critical target genes and pathways enacted by genomic alterations and lead to more accurately prediction of the effectiveness of novel cancer therapeutics and facilitate cancer research.
Historically, a number of tumour model systems have lacked scientific value due to the limited characterisation of genetic drivers, gene and protein expression, and in vitro/in vivo data. With these human melanoma cell lines, in vitro and in vivo modelling is brought into practice. The benefit of these established cell lines is that they were derived from patients’ tumours and thus represent the natural heterogeneity of this disease.
Melanoma cell lines – Features
These primary melanoma cell lines were derived directly from human melanoma tissue and resemble the tumour with all its heterogeneity and encompass detailed pathology, and clinical staging data of the patient. These models are biologically stable in terms of gene expression pattern, tumour architecture, and mutation status that allows for therapeutic targeting of known signaling pathways.
Multiple cell lines for each mutational subgroup allow for biological replicates within experiments, and primary and metastatic pairs allow for melanoma progression studies. These cell lines are validated by Short Tandem Repeats (STR) profiling, and by microarray gene expression analysis. These melanoma cell lines can metastasize to most organs of an immunodeficient animal (for example NOD/SCID mice).
Melanoma Cell Line – 2 formats available
These models have the ability to predict clinical activity as they are biologically stable in terms of gene expression pattern, tumour architecture and mutation status. These melanoma cell lines serve as individualised experimental models, important for drug treatment and outcome. This collection, originally provided by Dr. Meenhard Herlyn of The Wistar Institute, has been characterised to confirm a match to that of the originating patient tumour tissue via mRNA, qPCR and DNA short tandem repeat (STR) analysis. A summary table indicates the disease stage, gene mutations, pathology and relevant clinical data for each of these human melanoma cell lines. The key application of these cell lines include genetic studies, xenograft production, and drug testing and drug target discovery.
In addition to viable cells, non-viable cells for lysate production, genomic DNA, and total RNA from each melanoma cell line are also available.
Melanoma Cell Lines characterized for BRAF, N-RAS, KIT, PTEN and CDK4 mutations
Malignant melanoma is associated with genetic heterogeneity and a complex aetiology. In contrast to other skin cancers, melanoma has a strong tendency to metastasize with a consequently extremely poor overall prognosis for survival. The various phenotypes of melanoma are characterised by clinical features, such as bodily distribution or risk factors.
Cutaneous, uveal, acral, and mucosal melanomas have divergent clinical courses and are associated with distinct mutations, and risk factors. In the majority of melanomas, a number of genes and signaling pathways are involved in cellular proliferation and growth, and have been implicated in the pathophysiology of melanoma.
The collection of melanoma cell lines is organized based on the presence and/or absence of mutated or wild type genes. At a minimum, these cell lines have been characterised for the following genes: BRAF, N-RAS, KIT, PTEN and CDK4.
Quality – thoroughly tested melanoma cell lines
Research data reproducibility is dependent on high quality reagents for discovery, including melanoma cell lines. Each cell line developed is thoroughly tested for gene expression pattern, tumour architecture and mutation status. Cell lines are also tested for sterility, mycoplasma, mRNA, qPCR and DNA short tandem repeat (STR) analysis.
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