Live cell imaging of Actin, Tubulin & DNA – SLAS conference

SiR stain user's experiences to stain actin - tubulin

In the autumn of 2014, we presented new stains to conduct live cell imaging of Actin and Tubulin, which I covered in my post 2 new Actin and Tubulin live-cell imaging stains – without transfection!

SiR DNA picture 3 - speroid

Fig 1: Live MCF10A cells spheroid stained with SiR-DNA and imaged by confocal microscopy. Courtesy of C. Conrad and K. Jechow (Heidelberg).

SiR-Actin and SiR-Tubulin exhibit a number of benefits which have since then be experienced by a huge number of labs all over the world:

  • No transfection
  • No washing steps
  • No toxic effects if used in the concentration range recommended
  • Excellent brightness
  • Far-red excitation & emission
  • Deep tissue penetration and minimal background
  • Multiple fluorescent stainings with other dyes possible
  • Compatible with Superresolution microscopic techniques

Since then, Spirochrome has added another stain with the same benefits: SiR-DNA, a far-red, fluorogenic, cell permeable and highly specific probe for DNA (see Fig 1).

SLAS – High Content Screening Conference

From June 27th to June 29th, the SLAS – High Content Screening Conference took place in Dresden/Germany. The conference focussed on new essays and novel microscopy techniques.

Spirochrome and tebu-bio were selected to present a poster about the features of SiR-stains, which are excellent tools for phenotypic screening and high content screening (HCS) approaches.

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If you are interested in learning more about these exciting live cell imaging tools, don’t hesitate to download our joint poster:

Fluorogenic probes for live-cell imaging of intracellular targets


Any questions or comments? Please feel free to contact me with the form below!

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Written by Ali El Baya, PhD
Ali el Bayâ part of our Customer Success Team.