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Research areas
    ADME-Tox
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tebu-bio's blog - Acting and reacting in life sciences and biotechnologies
  • Home
  • Research areas
    • ADME-Tox
    • Biomarkers
    • Cell Biology and Signalling
    • Cell Sourcing – Cell Culture Technologies
    • Drug Discovery
    • Gene Expression – Molecular Biology
    • Stem Cells
    • Supplying Discovery Tools
  • Contact us
  • Meet the authors
  • GENE EXPRESSION & MOLECULAR BIOLOGY
    Gene editing TUNR system to remix the gene expression level
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  • STEM CELLS
    Pluripotent Stem Cells and iPSC Research: Quality and Innovative Products
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  • LIVE CELL IMAGING
    Live Cell Imaging - Watch SiR-Tubulin stained Human Osteosarcoma cells in action
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Supplying Discovery Tools

Adenovirus-based KO

22/11/2019 by Dimitri Szymczak, PhD No Comments

Knock-out of a gene of interest is a useful gene editing strategy to validate a gene function, to develop a synthetic lethality model or directly mimic a cancer cell in drug screening. Unfortunately, non-dividing cells and hard-to-transfect cells are a major challenge. However, there is an ideal solution that deserves to be more popular, the use of adenovirus.

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Supplying Discovery Tools

New – you know CRISPR, now it’s Prime Editing

28/10/2019 by Dimitri Szymczak, PhD No Comments

This month, a new leap forward in gene editing has been published in Nature under the title ‘Search-and-replace genome editing without double-strand breaks or donor DNA’. DOI: 10.1038/s41586-019-1711-4. Let’s take a look at prime editing to get a clear overview.

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Supplying Discovery Tools

Five new fluorescent probes to easily image live cellular processes

24/10/2019 by Frédéric Samazan No Comments

Real time visualization of cellular events by Live Cell Imaging (LCI) has led to a better understanding of the global dynamic biological process. In a constantly evolving field, tebu-bio regularly introduces innovative tools, such as this selection of 5 new LCI probes.

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Supplying Discovery Tools

Gene knock-out in hard-to-transfect cells

22/10/2019 by Dimitri Szymczak, PhD No Comments

Gene knock-out, which is gene editing leading to loss of function, just requires delivering into cells the 2 CRISPR system actors: the CAS9 endonuclease and the specific guide RNA to target the gene of interest. Thus, simple transfection of CleanCap CAS9 mRNA and gRNA followed by cell isolation with the smart aliquotor is a convenient and robust method. Howevery, what can you do if the cells are hard-to-transfect?

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Top contributors

Dimitri Szymczak, PhD
Dimitri Szymczak, PhD
  • Adenovirus-based KO
  • New – you know CRISPR, now it’s Prime Editing
Frédéric Samazan
Frédéric Samazan
  • Five new fluorescent probes to easily image live cellular processes
  • Immuno-Oncology: 6 must-haves to boost your projects
Isabelle Nobiron, PhD
Isabelle Nobiron, PhD
  • Accelerate your Biomarker discovery with High Density arrays
  • Want to know more about your Biologics?
Isabelle Topin, PhD
Isabelle Topin, PhD
  • Antibody Purification: Tips and Tricks
  • Monoclonal antibodies – all you need to know about antibody generation
Philippe Fixe, PhD
Philippe Fixe, PhD
  • ChIP for sensitive & fast genomic mapping
  • Recent developments in 3D cell culture hydrogel

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