Immunoassay development: reliable sources of quality reagents

We understand how crucial it is to have access to sustainable, quality sources of reagents when developing and/or producing the same immunoassays over long periods of times. Over the years, we’ve established partnerships with a number of experienced and renowned manufacturers, whose high-quality reagents we provide to researchers throughout the whole of Europe.

Immunoassay reagents – ease of supply, quality, consistency, bulk capacities and more…

It’s important to be able to find precisely what you need, ideally from a broad portfolio of immunoassay reagents to prepare and examine specimens, for a broad range of research and diagnostic applications.

At tebu-bio, our offer includes anti red-blood cell antibodies, secondary conjugated/unconjugated antibodies, gamma globulins, buffers, stabilizers, diluents and substrates to be used in the most common applications such as ELISA and Western Blot. You’ll also be able to select validated monoclonal antibodies against biomarker proteins that can be used as analyte specific reagents and are available for licensing.

Blocking reagents and controls

Blocking reagents are essential to improving assay sensitivity by reducing background interference and increasing signal-to-noise ratios. We provide high-quality blood products, including serum & plasma. Purified gamma globulin fractions can be used as blocking reagents as well as quantitation standards and assay loading controls.

Bulk quantities as large as over 1kg (if we take the example of sheep gamma globulin – Rockland Inc.) can be produced with your specific QC requirements, for use as a raw material in in vitro diagnostic kits. Test vial validation and long term reservation can all be arranged for your peace of mind.

The table below introduces some examples of the most popular catalogue blocking reagents and control we supply. You can get in touch with me for any bulk or customization requirement. We can fully adapt to your specific need, whether for specific concentrations, buffers, labelling, vialling etc…

Reagents & ControlsCatalog nr.
Goat SerumB304
Mouse SerumB308
Goat Gamma GlobulinD604-1000
Rabbit Gamma GlobulinD610-50
Rabbit Anti-Human Serum Albumin Antibody600-401-033
Rabbit Anti-Human RBC Antibody209-4139
Rabbit Anti-Sheep RBC Antibody213-4139
Red Blood Cell Depletion Magnetic Beads01-1801-50
Guinea Pig Complement (Fresh Frozen)C300-0100

Reporter molecules and conjugates

Secondary antibodies and Protein A/G conjugates are common and economical methods for detecting and visualizing the antigen-primary antibody complex. The streptavidin-biotin system is an alternative to secondary antibodies and provides significant signal amplification for conjugated primary antibodies. Our manufacturer’s strong experience allows for bulk production of highly purified reporter molecules and their conjugates, reducing concerns of lot-to-lot consistency.

Reporter Molecules & ConjugatesCatalog nr.
Goat Anti-Human IgG (H&L) Pre-adsorbed609-101-123
Rabbit Anti-Human IgM (Fc5µ)609-4131
Goat Anti-Mouse IgG (H&L) Pre-adsorbed610-101-121
Goat Anti-Rabbit IgG (H&L) Pre-adsorbed611-101-122
Donkey Anti-Goat (H&L) Pre-adsorbed605-701-125
Streptavidin ProteinS000-01
Protein A PA00-00
Note: Enzyme and Fluorochrome Conjugates also available

Solutions, stabilisers and substrates

We also offer supporting reagents to facilitate various immunoassays. Solutions, buffers, and stabilizers help maintain physiological conditions and promote protein-protein interactions. Substrates for reporter enzymes commonly used in immunoassays such as ELISA, FISH, Multiplex Western Blot… can produce soluble-colored or insoluble-colored products.

  Solutions, Stabilizers, & Substrates  Catalog nr.
TMB One Component HRP Microwell SubstrateTMBW-0100-01
BSA – Fraction VBSA-1000
BlockOut™ Universal Blocking BufferMB-073
ELISA Microwell Blocking BufferMB-064-1000
BCIP/NBT Alkaline Phosphatase SubstrateNBT-100
10X PBS Fish gel (NaN3 & Hg free)MB-066-1000

Validated antibody pairs for research use or commercial application

Rapid, point-of-care (POC) in vitro diagnostics tests that are easy to use have been largely sought after for their convenience, portability, and quick turn-around times allowing for faster decision making in regards to treatment and disease control. Diagnostic assays that directly identify an infectious agent (Lateral flow methods) or disease biomarker have become increasingly essential to managing patient treatment or facilitating pathology diagnostics. For this purpose, we are able to bring you an exhaustive offer of matched antibody pairs (Meridian Life Sciences) together with their control antigens to provide a most exhaustive range of antibodies and proteins for assay development.

This thorough brochure will allow you to identify all available pairs for the pathology you are working on, from infectious disease antigens, to cancer and cardiovascular markers, auto-immune diseases and veterinary pathologies.
This will save you the time to look for matching antibody pairs in house for your immunoassay. It’s already been done for you.

I take this opportunity to inform you of the latest releases of readily available recombinant COVID19 proteins in various expression systems, from insect cells to HEK293, E. coli to CHO

All reagents are available either for research use or commercial application with no royalties attached.

Written by Isabelle Nobiron, PhD
Isabelle is a Product Manager at tebu-bio, and also the company's ISO Quality Manager.