How to get rid of fibroblastic contaminating cells?

Tissue culture for Primary cell isolation are often jeopardized by the outgrowth of contaminating fibroblasts. As fibroblasts usually grow at a much faster rate compared to the target cell population, their expansion has to be tightly controlled to end up with a culture as pure as possible.

Any solutions in the literature?

A search reveals that there are numerous published efforts to inhibit fibroblast outgrowth and this process appears to be tissue specific.  For instance, the addition of melatonin to melanocyte preparations potently inhibits the proliferation of fibroblasts that were passed along in the isolation procedure from whole human skin.  Another example is that of adenosine and its inhibition of collagen and protein synthesis in cardiac fibroblasts.  Specific antibodies can act as inhibitors of fibroblasts growth and proliferation.  A DNA topoisomerase inhibitor (camptothecin) can be included in culture medium to eliminate non-neuronal cell proliferation in neuron-type primary cell isolation.  In addition, it has been reported that D-valine is a selective agent for rat epididymal epithelial cells, but not for rabbit epithelial cells, and that cytosine arabinoside is a simple and effective means to control the proliferation of fibroblast-like cells in both rat and rabbit epididymal cell cultures.

Together, a tissue-specific optimal fibroblast inhibitory system can be developed to improve the performance of primary cell isolation.

Remove unwanted cells with FibrOut™

FribrOut - prevent overgrowth of fibroblastic and contaminated cellsThe fibroblast growth control system, FibrOut™ developed by CHI Scientific is a proprietary combination of several biochemical compounds, antibodies, or specialty reagents, which prevent overgrowth of fibroblastic or contaminating cells, effectively increasing the yield of target cells. Each fibroblast growth inhibitory system is customized to be tissue-specific and cell-specific.

Each FibrOut™ system may contain one or more of the following chemicals (1) Trypsin; (2) Collagenase; (3) D-valine; (4) Cis-OH-proline; (5) Ethylmercurithiosalicylate; (6) Phenobarbitone, (7) Formulated serum substitutes. Some of the systems also contain antimesodermal antibodies.

FibrOut™ is available throughout Europe from tebu-bio, get in touch if you have any questions or need to optimize your Primary cell culture, we’ll be pleased to help!

Written by Isabelle Nobiron, PhD
Isabelle is a Product Manager at tebu-bio, and also the company's ISO Quality Manager.