How can you get your mRNA ready in only a few weeks?

Synthetic mRNA is indeed the highway for today’s therapeutics, whether you’re working on developing new drugs against cancer targets, or whether you aim to develop new vaccines, Covid-19 being one of them. So, how can you obtain your mRNA within just a few weeks, ready to explore these potential applications? Read on to see how we can help you achieve this.

European provider for your RNA-based research

All you need to do is to give us your ORF sequence, from ATG to stop codon. We will do the rest.

Within 5 to 8 weeks, depending on the size of your ORF, you will receive your tailored mRNA with the following features.

Capping with CleanCap

  • +96% functional mRNA
  • High yield in mgs
  • Superior protein production
  • Natural mammalian CAP1
  • CleanCap set a news standard for superior mRNA-based protein production in vivo

How does it work?

CleanCap and optimal template for Mammalian cells

TriLink Biotechnologies, a Maravai LifeSciences company, has developed the CleanCap AG reagent and built templates including proprietary UTRs for optimal translation into Mammalian cells. tebu-bio is their unique distributor in Europe.

These templates ensure accurate transcription of a polyA tail of 120A. A T7 promoter, compatible with the CleanCap AG reagent, provides +96% capping. Its structure corresponds to the natural CAP1 found into Mammalian cells.

We also offer eGFP ready-to-use mRNA with U-reduction and 5moU, for you to assess the value of such modifications, using the right controls. U reduction followed by codon optimization improves RNA yields during in vitro transcription as well as protein expression in vivo.

Frequently asked questions

Q. Is it possible to use my own UTRs?

A. Yes, it is. For each mRNA production of 1ml transcription scale, you just need to provide >180µg of your own plasmid template including your UTR. Just let us know which enzyme should be used for linearization (it must be positioned after the DNA sequence corresponding to the mRNA). You should also indicate if your template includes a sequence for the polyA tail or if enzymatic polyA tail synthesis is required.

Just keep in mind that it is not more costly to use our own optimized sequences (it may even be cheaper). This solution may be much simpler for you: no need to send us your plasmid, you send provide your sequence and we do the rest. You rely on experts to get the best yields from this transcription.

By the way, do you know that tebu-bio laboratories can do the plasmid preps for you? Learn more here!

Q. I know what my prefered UTRs sequences would be but I have no template ready yet. What can you do for me?

A. We can build the template for you. Typically we could add a T7 promoter sequence compatible with CleanCap AG reagent, your UTRs and a 80A polyA tail to a pUC57.

Q. In which buffer and final concentration will my mRNA be delivered?

A. By default the concentration will be around 1mg/ml, packaged as a solution in 1 mM Sodium Citrate, pH 6.4. However, you can ask for another buffer and/or another concentration without any extra fee. I would still recommend a storage at a minimum concentration of 1mg/ml to ensure optimal RNA stability.

Q. How should I store my mRNA?

A. The mRNA should be stored at -80°C, avoiding freeze/thaw cycles. It is therefore recommended to aliquot it. We can prepare aliquots for you upon request.

Q. How can I move forward to a production scale suited to clinical trials?

A. The production scale illustrated in this post is for research use only. However, the same manufacturer we collaborate with has GMP facilities and can provide GMP mRNA bulk synthesis. We can assist you with this transition.

Q. My ORF sequence is highly confidential. How can I be sure my data will be securely processed?

A. tebu-bio handles your data under strict confidentiality procedures. We can of course arrange to sign a CDA if required. Feel free to provide us with your own template for this.

Q. What is your recommendation to deliver the mRNA into the cells?

A. The development of delivery vehicles for therapeutics perspectives is a project by itself. There are some expert companies working on this. For research applications, and notably for proof of concept of the gene expression, I would suggest the high grade and LNP-based transfection reagent called, mFect. It was also tested successfully by injections into mice. Furthermore, control mRNA such as eGFP or luciferase coding mRNA should be tested to monitor the delivery solution.

Q. Can I produce my custom mRNA and still benefit from this high capping efficiency solution?

A. Yes, you can. We also provide the CleanCap AG reagents and the technical support to go with it to allow you to do so.

Any more questions we haven’t answered here?

Written by Dimitri Szymczak, PhD
Dimitri Szymczak is a Technical Support Specialist and Product Manager at tebu-bio, and a fan of capoeira in his spare time.