Tetramethylbenzidine (TMB) is a classic substrate for peroxidase activity that is used for microwell plate analysis such as an ELISA test. A quantitative color change from yellow to blue reveals the activity and can be read at 620-650 nm for ELISA test.

Substrate selection in immunoassay development

Surmodics have developed several types of TMB, for different purposes.

ELISa test with several type of TMB
Several TMB to fine tune immunoassay development

TMBW is the gold standard with fast reaction.

TMBS shows super high sensitivity (not shown on the previous image).

TMSK is the slow kinetics, TTMB is the super slow kinetics and TMBX offers an extended range.

For membranes instead of microwell plates, there are dedicated types of TMB. They can be very useful for Western blot without the need for a dark room. They are the standard TMBM and the enhanced version ESPM to catch low signals.

Best-in-class TMB

We provide TMB microwell substrate manufactured by Surmodics. It is of gold standard, which ensures low background, stability, sensitivity and lot-to-lot consistency.

TMB performances
TMBW and TMBS are the best substrates for ELISA test
Consistency of Surmodics TMB
Lot-to-lot consistency of the TMB to improve data reproducibility

Convenient use and storage

Developed as single-component, Surmodics TMB is easy to use. It’s also stable for years and so compatible with long term storage.

TMBW stability
Highly stable one component TMB

Taking in account the fact that our TMB components are also price competitive, they are the top choice for optimal results in your ELISA test!

Written by Dimitri Szymczak, PhD
Dimitri Szymczak is a Technical Support Specialist and Product Manager at tebu-bio, and a fan of capoeira in his spare time.